摘要
目的:观察胰岛素对人视网膜微血管内皮细胞syndecan-1的表达及细胞的增殖和通透性的影响。方法:以低浓度(100nmol/L)和高浓度(1000nmol/L)胰岛素分别刺激细胞48h后,采用蛋白印迹和实时定量聚合酶链反应观察syndecan-1蛋白和mRNA的表达变化。分别以四甲基偶氮唑蓝法和辣根过氧化物酶法观察细胞的增殖性和通透性。结果:不同浓度的胰岛素刺激后,视网膜微血管内皮细胞syndecan-1蛋白和mRNA的表达均升高,且高浓度胰岛素的作用更显著。胰岛素刺激后,视网膜微血管内皮细胞的增殖水平和通透性均增高,且高浓度胰岛素的作用更强。结论:胰岛素可上调人视网膜微血管内皮细胞syndecan-1的表达、增加细胞的通透性和促进细胞的增殖。
AIM： To investigate the effects of insulin on syndecan-1 expression, cellular permeability and proliferation in human retinal microvascular endothelial cells. METHODS： Cells were treated with 100 nmol/L and1000 nmol/L insulin for 48 h respectively. Expression of protein and mRNA were detected by western blot and quantitative real-time polymerase chain reaction.Cellular proliferation and permeability were examined by methods of methylthiazolyl tetrazolium and horseradish peroxidase. RESULTS： With treatment of insulin, protein and mRNA of syndecan-1 both increased obviously,and the effect of high level insulin was more significant. After treated with insulin, cellular proliferation and permeability both enhanced,and the effects of high level insulin were stronger.CONCLUSION： Insulin can up-regulate syndecan-1 protein and mRNA in cultured human retinal microvascular endothelial cells, and increase cellular permeability and proliferation.
作者
胡永亮
王静波
周历
韩晓霞
师凌昊
Yong-Liang Hu;Jing-Bo Wang;Li Zhou;Xiao-Xia Han;Ling-Hao Shi(Department of Dermatology;Department of Ophthalmology,the 309^th Hospital of Chinese PLA,Beijing 100091,China;Department of Ophthalmology and Otorhinolaryngology,the 93735 ^th Hospital of Chinese PLA,Tianjin 301700,China)
出处
《国际眼科杂志》
CAS
北大核心
2018年第8期1381-1384,共4页
International Eye Science
基金
北京市自然科学基金(No.7173269)
国家自然科学基金(No.81100684)
首都卫生发展科研专项(No.2011-5007-02)
总参军事医学和老年病科研基金项目(No.ZCWS14C19)
