摘要
本研究建立了一种液相色谱测定茶皂素含量的方法。色谱柱SUPELCOTM LC-18(4.6 mm×250mm,5μm);流动相为甲醇-0.1%磷酸(体积比40:60);流速0.8 m L/min;检测波长267 nm;柱温30℃。该方法平均加标回收率90.88%~113.13%,精密度达0.19%,定量检出限0.67%,测得水相固形物中茶皂素质量分数为54%,与比色法测定结果相接近,但液相法具有测定简便、快速、准确等特点;与相关液相测定茶皂素方法报道相比,该方法色谱峰分离较好,且避开了溶剂峰干扰等特点,适合作为测定茶皂素含量的分析方法。
A liquid chromatographic determination method of saponin content was established in this article. This method average recovery rate of saponin was 90.88-113.13%, the degree of precision was 0.19%, the quantitative detection limit was 0.67%, and the content of saponin from water phase solids was about 54%, when the determination conditions were as follows： SUPELCOTM LC-18 （4.6＊250mm, 5μm）, mobile phase methanol-0.1% phosphoric acid solution （ volume ratio 40：60 ）, flow rate 0.8mL/min, detecting wavelength 267nm, column temperature 30℃. Although the liquid chromatographic method result of saponin content was similar to the spectrophotometric method, this method was simplicity, quickness and accuracy. And this liquid chromatographic method had a betterSseparationSeffect on chromatographSpeaks, and could avoid the solvent peak interference, when compared with the relevant report on liquid chromatographic method of saponin content. ItSwasSa suitable analyzesSmethodSfor determination of saponin content.
作者
郭咪咪
薛雅琳
张东
段章群
王雅朦
朱琳
李秀娟
李晓宁
GuoMimi;Xue Yalin;Zhang Dong;Wang Yameng;Zhu Lin;Li Xiujuan;Duan Zhangqun;Li Xiaoning(Oil and Fat Chemistry Research Group,Academy of State Administration of Grain,Beijing 100037)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2018年第7期105-109,共5页
Journal of the Chinese Cereals and Oils Association
基金
公益性行业(粮食)科研专项(201513003-7)
关键词
水相固形物
茶皂素
含量
液相色谱
water phase solids
saponin
content
liquid chromatographic
