摘要
采用平板对峙法和盆栽回接实验,探究回接根瘤菌对蚕豆的生长影响及根腐病的抗性效应。结果表明:根瘤菌SWFU55-1、SWFU55-2、SWFU48-1对镰孢菌XM20生长出现不同程度的抑制,抑菌率分别为55%、38%、40%;根瘤菌对蚕豆的生长有显著的促进作用,平均冠幅、平均分蘖数、平均根瘤数、平均结荚数、植株含氮量等各项指标均表现为SWFU48-1>SWFU55-1>SWFU55-2>空白组>接种XM20组;3株根瘤菌的抗性评价也同样表现为SWFU48-1(相对防治效果49.49%)>SWFU55-1(相对防治效果20.36%)>SWFU55-2(相对防治效果7.73%),SWFU55-1、SWFU55-2、SWFU48-1对XM20有一定的拮抗作用,对蚕豆生长具有促进作用,根瘤菌回接能有效提高蚕豆的抗病性,减少叶片变黑的比率,减轻病害的为害程度。
By confront culture on plate and pot experience, Vicia faba was taken as material to investigate the inoculation effect of Rhizobia on the growth of V. faba and its disease resistance on root rot. The confront culture results show that SWFU55-1, SWFU55-2, SWFU48-1 inhibited the growth of XM20 in different levels, the bacteriostatic rates were 55%, 38% and 40%, respectively. Inoculation pot experiments show that Rhizobia had a significant promoting effect on the growth of V. faba. Effect analysis show that SWFU48-1 〉 SWFU55-1 〉 SWFU55-2 〉 CK 〉 XM20 inoculation group on the average crown width, average tiller number, average nodule number, average pod number and nitrogen content. The resistance evaluation results also show that SWFU48-1 （relative control effect is 49. 49%） 〉SWFU55-1 （relative control effect is 20.36%） 〉SWFU55-2 （relative control effect is 7.73%）. SWFU55-1, SWFU55-2, SWFU48-1 have a certain antagonism on XM20, which has a significant promoting effect on the growth of V.faba, Rhizobia inoculation can effectively improve the disease resistance of V.faba, reduce the ratio of leaf blackened, decrease the damage of the disease degree.
作者
王金华
聂中良
徐玉巧
郑毅
Wang Jinhua;Nie Zhongliang;Xu Yuqiao;Zheng Yi(College of Plant Protection,Yunnan Agricultural University,Kunming Yunnan 650201,China;College of Life Sciences,Southwest Forestry University,Kunming Yunnan 650224,China;Yunnan Provincial Education Bureau,Kunming Yunnan 650223,China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2018年第4期94-99,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
国家自然科学基金(31100007)资助
生物学一级学科(51400666)资助
关键词
根瘤菌
镰孢菌
根腐病
蚕豆
生长
抗病
Rhizobia
Fusarium oxysporum
root rot
Vicia faba
growth
disease resistance
