摘要
基因编辑技术(Gene Editing)能够让人类对目标基因进行"编辑",实现对特定DNA片段的敲除、敲入等操作。目前基因编辑技术主要有RNA干扰(RNAi)、锌指核酸酶(ZFNs)、转录激活子样效应因子核酸酶(TALEN)和成簇的规律性间隔的短回文重复序列(CRISPR)等,基因编辑技术已经被广泛应用在线虫、果蝇、拟南芥、小鼠和斑马鱼等模式生物的基因组学、遗传发育、基因功能研究中。随着水产生物全基因组测序工作的开展,水产生物后基因组时代到来,基因编辑技术在水产生物领域应用得到较快发展,文章从基因编辑技术原理、技术发展和基因编辑技术在水产生物基因功能、遗传发育、性别调控及基因调控等方面研究进行综述、进而对基因编辑技术在水产生物中的研究前景进行了展望,旨在为更好地开展水产生物的基因功能和基因调控网络研究提供参考。
Gene Editing can be used to operate artificially almost all gene the specific fragments of DNA knockout and knockin operation in cells or organisms. At present, gene editing technology mainly including RNA interference（RNAi）, zinc finger nuclease（ZFNs）,Transcription activator like effector nuclease（TALEN） and Clustered regularly interspaced short palindromic repeats（CRISPR）.Gene editing technology has been widely applied in Celegans, Drosophila, Arabidopsis, mouse and zebrafish genomics, genetic development, gene function study. With the development of genome sequencing of aquatic organisms and the advent of the post genome era of aquatic organisms, the technology of gene editing has developed rapidly in the field of aquatic organisms. In this article, the gene editing technology principle, technology development, gene function and genetic sex regulation were reviewed in aquaculture field. At same time research prospect of the gene editing technology in aquatic organisms. In order to provide inspiration and reference for the study of gene function and gene regulation network in aquatic organisms.
作者
王峰
李忠
苗贵东
WANG Feng;Li Zhong;MIAO Guidong(Guizhou Provincial Key Laboratory of Chemical Synthesis and Environmental pollution control and remediation,Xingyi Guizhou 562400;Guizhou Provincial Key Laboratory for Research and Development of National Medicinal Biological Resources,Xingyi Guizhou 562400;Xingyi Normal University for Nationalities,Xingyi Guizhou 562400)
出处
《兴义民族师范学院学报》
2018年第3期112-121,共10页
Journal of Minzu Normal University of Xingyi
基金
贵州省科技厅联合基金项目:黔科合[2016]7040号
黔科合[2017]7021号
兴义民族师范学院博士启动基金:16XYBS16
国家级大学生创新创业计划项目(201710666001
201710666005
201710666043)
贵州省大学生创新创业计划项目(201710666011)
关键词
水产生物
基因编辑
RNA干扰
人工核酸酶技术
研究进展
Aquatic organisms
Gene edit
RNA interference
Artificial nuclease technology
Research progress
