摘要
目的:探讨当归补血汤对顺铂损伤大鼠卵巢颗粒细胞凋亡蛋白以及氧化损伤的影响。方法:以不同剂量的当归补血汤含药血清作用于5μg/m L顺铂(Cisplatin,CDDP)抑制的体外培养的大鼠卵巢颗粒细胞,用MTS[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]法检测各组颗粒细胞24 h、4 8 h、72 h增殖情况,及培养液中丙二醛(Malondialdehyde,MDA)和胱天蛋白酶-3(Caspase-3)蛋白表达情况。结果:CDDP对大鼠卵巢颗粒细胞有明显抑制作用,凋亡率可以达到70.50%。当归补血汤含药血清对颗粒细胞增殖有促进作用,2.5%当归补血汤含药血清作用4 8 h组最为显著(P<0.01);同时MDA和caspase-3蛋白也逐渐降低,差异有统计学意义(P<0.01)。与当归补血汤低、高剂量治疗组比较,当归补血汤中剂量治疗组效果最明显,差异有统计学意义(P<0.01)。结论:当归补血汤对顺铂损伤大鼠卵巢颗粒细胞增殖有促进作用,可能机制是抑制细胞氧自由基的产生,抑制Caspase-3蛋白表达,促进细胞增殖。
Objective：To discuss the effect of medicated serum of Dangui Buxue tang on apoptosis protein and oxidative damage of ovarian granulosa cells in rats with Cisplatin injury. Methods： Different dosage of medicated serum of Dangui Buxue tang was applied to ovarian granulosa cells in rats in vitro inhibited by 5 μg/m L Cisplatin（CDDP）. MTS（3-（4, 5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2 H-tetrazolium） method was used to detect the proliferation of granulosa cells in each group at the 24 th,48 th and 72 nd hour,Malondialdehyde（MDA） and the expression of Caspase-3 protein in culture medium. Results：CDDP had obvious inhibitory effect on ovarian granulosa cells in rats,and the apoptosis rate can reach 70.50%. Medicated serum of Dangui Buxue tang can promote the proliferation of granulosa cells,and the effect of 2.5% medicated serum of Dangui Buxue tang on the 48 th hour group was the most obvious（P〈0.01）;MDA and Caspase-3 protein decreased gradually at the same time, the difference being significant（P〈0.01）. Compared with that in the Dangui Buxue tang groups of low-dose and high-dose,the curative effect in the Dangui Buxue tang group of middle-dose was the most obvious, the difference being significant（P〈0.01）. Conclusion： Dangui Buxue tang can promote the proliferation of ovarian granulosa cells in rats with Cisplatin injury. Its mechanism probably is to inhabit the production of oxygen free radicals of cells and the expression of Caspase-3 protein,and promote the proliferation of cells.
作者
赵粉琴
徐世倩
袁爱倩
李娜娜
ZHAO Fenqin;XU Shiqian;YUAN Aiqian;LI Nana
出处
《新中医》
CAS
2018年第7期8-11,共4页
New Chinese Medicine
基金
甘肃省自然科学基金项目(148RJZA072)
甘肃中医学院中青年基金项目(2305016601)
