摘要
【目的】分析牦牛肉中稳定碳、氮、氢同位素组成,以及它们受地域、牧草和饮水的影响,为其产地溯源及真伪鉴别提供技术支撑。【方法】从青海省海北、海南、玉树3个不同地域采集牦牛肉、牧草及水样品,利用GPS定位采样地的经度、纬度及海拔高度,利用元素-稳定同位素比率质谱仪(EA-IRMS)分析样品中稳定碳、氮、氢同位素比率。【结果】牦牛脱脂肌肉、粗脂肪中的δ^(13)C平均值分别为(-23.99±0.25)‰和(-28.77±0.50)‰;脱脂肌肉中的δ^(15)N和δ~2H平均值分别为(4.04±0.91)‰和(-107.99±11.08)‰。牦牛肉中δ^(13)C、δ^(15)N值主要受其食用的牧草影响,地域对牦牛肉中的δ^(13)C值也有一定的影响,即牦牛肉中δ^(13)C值有随海拔的增加而增加的趋势。青海省海北、海南、玉树3个地区牧草和水中的δ~2H值均有极显著差异,牧草中δ~2H值由高到低的顺序依次为海南>海北>玉树,水中δ~2H值由高到低的顺序依次为海北>海南>玉树。牦牛肌肉中δ~2H值由高到低的顺序依次为海南>海北>玉树,与牧草中δ~2H值的地域变化顺序一致。说明牦牛肉中的δ~2H值与牧草、饮水密切相关,均有随海拔升高而降低的趋势,且牧草对牦牛组织中氢同位素组成的影响可能大于水的影响,但这还需要进一步研究证实。【结论】牦牛肉中稳定同位素指纹与高海拔地区的牧草、饮水、地形密切相关,具有独特的指纹特征。
【Objective】The characters of carbon, nitrogen and hydrogen isotopic composition in yak（Bos grunniem） muscle and the effect of geographical origin, pasture and drinking water were analyzed, which would provide the technical support for tracing and identifying the yak meat. 【Method】The yak muscle, pasture and water samples were collected from Haibei, Hainan and Yushu of Qinghai Province, and longitude, latitude and altitude of sampling site were recorded by using GPS. The carbon, nitrogen and hydrogen stable isotopic ratio in samples were tested by using EA-IRMS. 【Result】The average value of δ^（13）C in yak de-fatted muscle and fat was（-23.99±0.25）‰ and（-28.77±0.50）‰, respectively, and the average value of δ^（15）N and δ~2H in yak muscle was（4.04±0.91）‰ and（-107.99±11.08）‰, respectively. The δ^（13）C and δ^（15）N values in yak muscle were closely related to the pasture of the Tibetan Plateau, and the region had certain effect on δ^（13）C values of yak muscle, indicating the δ^（13）C values increased with the altitude increasing. The δ~2H values of pasture and water samples among three regions of Haibei, Hainan and Yushu of Qinghai Province showed significant differences. The order of δ~2H values in pasture was HainanHaibeiYushu, and the order of δ~2H values in water was HaibeiHainanYushu. The order of δ~2H values in yak muscle was HainanHaibeiYushu, and it was the same order as the pasture. The results indicated that the δ~2H values in yak muscle were closely related to the drinking water and pasture, and they were decreased with altitude increasing, and the pasture probably had more effect on δ~2H values in yak muscle than water. But it needs to be further researched and confirmed.【Conclusion】The stable isotopic fingerprints in yak muscle were closely related to the pasture, drinking water and terrain of high altitude, which had the unique fingerprints character.
作者
郭波莉
魏益民
魏帅
孙倩倩
张磊
师振强
GUO BoLi;WEI YiMin;WEI Shuai;SUN QianQian;ZHANG Lei;SHI ZhenQiang(Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences~Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture, Beijing 100193)
出处
《中国农业科学》
CAS
CSCD
北大核心
2018年第12期2391-2397,共7页
Scientia Agricultura Sinica
基金
西藏自治区重点科研项目(ZD20170014)
关键词
牦牛肉
稳定同位素指纹
牧草
饮水
yak muscle
stable isotope fingerprints
pasture
drinking water
