摘要
目的探讨血清miR-765与CA153联合检测对乳腺癌的诊断价值。方法收集乳腺癌患者58例,乳腺良性疾病患者28例及体检健康者30例,应用实时荧光定量聚合酶链反应检测血清miR-765的表达水平,受试者工作特征曲线分析单一和联合检测对早期乳腺癌的诊断价值。结果乳腺癌患者血清miR-765相对表达量均显著低于乳腺良性疾病患者和健康对照者(F=35.97,均P<0.01);单独检测miR-765的ROC曲线下AUC为0.843(95%CI:0.767~0.919),灵敏度为87.9%,特异度为81.0%;miR-765与CA153联合检测的ROC曲线下AUC为0.903(95%CI:0.844~0.962),灵敏度为89.7%,特异度为86.2%;CA153<25U/ml的乳腺癌患者血清miR-765的ROC曲线下AUC为0.840(95%CI:0.746~0.934),灵敏度为87.5%,特异度为82.4%。结论 miR-765对乳腺癌的诊断具有一定价值,与CA153联合检测可为临床提供一种潜在的辅助方法。
Objective To investigate the diagnostic value of serum miR-765 and CA153 in patients with breast cancer. Meth- otis 58 cases of patients with breast cancer,28 cases of patients with benign breast disease and 30 cases of healthy individu- als were collected. The level of serum miR-765 was determined by real-time fluorescence quantitation polymerase chain reac- tion （PCR）. Receiveroperating characteristic （ROC） curve was used to analyze the diagnosis value of single and combined detection in breast cancer. Results The relative expression levels of miR-765 in breast carcinoma patients were significantly lower than those in benign breast disease and healthy controls （F= 35.97, all P〈0.01）. The receiver operating characteristic（ROC） curve analysis of serum miR-765 for the diagnosis of breast cancer yielded AUC of 0. 843 （95 % CI：0. 767~0. 919） with 87.9% sensitivity and 81.0% specificity. The ROC curve analysis of combined miR-765 and CA153 for breast cancer detection yielded AUC of 0. 903 （95%CI：0. 844~0. 962） with 89.7% sensitivity and 86.2% specificity. The ROC curve a- nalysis of serum miR-765 as biomarkers for the group （CA153〈25 U/ml） of breast cancer yielded AUC of 0. 840 （95%CI： 0. 746~0. 934） with 87.5 % sensitivity and 82.4% specificity. Conclusion miR-765 showed that it would has certain diag- nostic value for breast cancer,and combined with CA153,miR-765 may provide a potential assistant diagnostic method.
作者
洪宏
袁建芬
喻海忠
HONG Hong;YUAN Jian-fen;YU Hai-zhong(Department of Clinical Laboratory, Nantong Hospital of Traditional Chinese Medicine , J iangsu Nantong 226001 ,China)
出处
《现代检验医学杂志》
CAS
2018年第3期92-94,共3页
Journal of Modern Laboratory Medicine
基金
南通市社会发展计划资助项目(MS2017017-4)
