摘要
目的探讨酪蛋白激酶2相互作用蛋白1(CKIP-1)基因对人胃癌细胞增殖、凋亡和周期的影响。方法培养人类低分化胃癌细胞株MKN45,转染过表达CKIP-1(过表达组)、干扰CKIP-1(干扰组)及空载质粒(空载组),观察三组细胞的增殖、凋亡和周期情况。结果过表达CKIP-1抑制细胞增殖,促进细胞凋亡;干扰后促进细胞增殖,抑制凋亡(P<0.05或P<0.01)。过表达组S期细胞比例高于空载组,G2/M期细胞比例低于空载组(P<0.05);干扰组S期细胞比例低于空载组,G2/M期细胞比例高于空载组(P<0.05)。结论 CKIP-1参与胃癌细胞的增殖、凋亡和周期过程,可能与胃癌的发生、发展有关。
Objective To investigate the effects of casein kinase 2 interacting protein 1（CKIP-1）gene on the proliferation,apoptosis and cell cycle of human gastric cancer cells.Methods The overexpressed plasmid（group A）,interfering plasmid（group B）and empty plasmid（group C）of CKIP-1 gene were transfected into human low differentiated gastric cancer cell lines MKN45 cells.The cell proliferation,apoptosis and cell cycle were observed.Results The overexpression of CKIP-1 inhibited cell proliferation and promoted cell apoptosis（P〈0.05 or P〈0.01）.Interference of CKIP-1 promoted cell proliferation and inhibited cell apoptosis（P〈0.05 or P〈0.01）.The percentage of S phase was higher and that of G2/M phase was lower in group A than that in group C（P〈0.05）.The percentage of S phase was lower and that of G2/M phase was higher in group B than that in group C（P〈0.05）.Conclusion CKIP-1 gene is involved in gastric cancer cell proliferation,apoptosis and cell cycle,which may be related to the occurrence and development of gastric cancer.
作者
王礼鑫
褚明亮
张著学
张赟
曹颖
官志忠
WANG Lixin;CHU Mingliang;ZHANG Zhuxue(Department of Pathology, Guizhou Medical University, Guiyang 550002, CHIN)
出处
《江苏医药》
CAS
2018年第6期609-611,共3页
Jiangsu Medical Journal
基金
贵州省科技厅基金项目(黔科合基础[2016]1085
黔科合J字[2015]2086)
贵州省人民医院博士基金(GZSYBS[2015]01)
贵州省科学技术厅
贵州省人民医院联合基金计划项目(黔科合LH字[2015]7108号
黔科合LH字[2016]7136号)
