摘要
Objective: To study the correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy. Methods: A total of 54 patients with pancreatic cancer who received radical resection and 32 patients with pancreatic trauma who received partial resection in Tianyou Hospital Affiliated to Wuhan University of Science &Technology between May 2014 and March 2017 were selected, proper amount of pancreatic cancer tissue and normal pancreatic tissue were collected respectively, RNA was extracted and synthesized into cDNA by reverse transcription, and then fluorescence quantitative PCR kits were used to determine TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression. Results: TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue were significantly higher than those in normal pancreatic tissue;Survivin, USP9X, DPF2 and Stathmin mRNA expression in pancreatic cancer tissue with high TWEAK expression were significantly higher than those in pancreatic cancer tissue with low TWEAK expression;CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue with high Del-1 expression were significantly higher than those in pancreatic cancer tissue with low Del-1 expression. Conclusion: The high expression of TWEAK and Del-1 in pancreatic cancer tissue can promote the proliferation and invasion of pancreatic cancer cells respectively.
