摘要
目的:研究补肾益气方诱导母胎界面耐受状态的机制及吲哚胺2,3-双加氧酶(IDO)对蜕膜NK细胞表型转化的影响。方法:用免疫组织化学方法测定正常绒毛组织和复发性流产患者绒毛组织内IDO的表达。体外培养人滋养细胞HTR-8/SVneo,用流式细胞术检测含药血清对HTR-8/SVneo IDO表达的影响。用含对照大鼠血清、含药大鼠血清及含药血清联合IDO阻断剂1-甲基色氨酸(1-MT)的培养液分别处理HTR-8/SVneo后与人外周NK细胞共培养,测定其对NK细胞的调节作用。结果:正常绒毛组织中IDO的表达较流产绒毛组织明显增加。含药血清可增强HTR-8/SVneo IDO的表达。外周NK细胞与含药血清处理过的HTR-8/SVneo共培养后,NK细胞表面CD16表达减低,CD56表达增加,且CD16的改变可被1-MT抑制。结论:补肾益气方可以通过上调滋养细胞IDO的表达诱导外周NK细胞向耐受型转变。
Objective：To study the mechanism of kidney-replenishing herb in regulating the tolerance of fetal-maternal interface and the effect of IDO on modulating the phenotype of decidual NK cells.Methods：Indoleamine-2,3-dioxygenase（IDO） expression in villus tissues from normal pregnancies and recurrent spontaneous abortion（RSA） patients was monitored by immunohistochemistry（IHC）.IDO expression in HTR-8/SVneo cells treated with control or herb serum medium was detected by flow cytometry（FCM）.The influence of kidney-replenishing herb on modulating the phenotype of NK cells,CD16 and CD56 expression in peripheral NK cells co-cultured with control or herb serum medium in the presence or absence of 1-methyltryptophan（1-MT） treated HTR-8/SVneo cells were measured by FCM.Results：IDO expression was decreased in villus tissue of RSA patients compared to normal pregnancies.Herb medium could increase the IDO expression of HTR-8/SVneo.Kidney-replenishing herb could enhance the regulatory function of trophoblasts on NK cells and further induce the immune tolerance at fetal-maternal interface in IDO dependent and independent manners.Conclusion：Kidney-replenishing herb can modulate the phenotype of peripheral NK cell by up-regulating IDO expression in trophoblasts and play a role in the treatment of RSA patients.
作者
杨少良
牛甜甜
李明清
王海燕
YANG Shao-Liang;NIU Tian-Tian;LI Ming-Qing;WANG Hai-Yan(Department of Gynecology of Integrated Traditional Chinese and Western Medicine, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第6期846-849,860,共5页
Chinese Journal of Immunology
基金
国家自然科学基金(No.81373868)
关键词
补肾益气方
IDO
母胎免疫耐受
Kidney-replenishing herb
IDO
Maternal-fetal immune tolerance
