摘要
目的观察Roux-en-y胃旁路术(RYGB)对2型糖尿病(T2DM)大鼠缓激肽受体2(B2R)和血管生成样蛋白2(Angptl 2)的影响。方法将60只SD大鼠随机分为糖尿病组40只和正常对照组(NC组)20只。糖尿病组大鼠给予高脂高糖饮食4周后,经腹腔注射链脲佐菌素(STZ)建立模型。将成模大鼠再随机分为实验组(RYGB组)15只(手术后死亡2只)和假手术组(DM组)15只。RYGB组大鼠行RYGB手术,DM组和NC组大鼠接受原位手术。三组大鼠分别于术前和术后4周测定三组大鼠的体重、FBG、FINS、ISI、Angptl 2及B2R水平。结果 RYGB组与DM组大鼠术前体重、FBG、FINS、ISI、ANGPTL2、B2R水平无明显差异(P>0.05)。术后4周RYGB组大鼠较术前体重、FBG、FINS均明显下降(P<0.05),ISI、ANGPTL2及B2R上升(P<0.05)。DM组、NC组大鼠术后4周的体重、FBG、FINS、ISI、ANGPTL2、B2R水平与术前比较,差异无统计学意义(P>0.05)。结论胃转流术可能是通过降低T2DM大鼠B2R和Angptl2的水平,进而达到减重、降低血糖的效果。
Objective To research the influence of Roux-en-y gastric bypass surgery on bradykinin receptor 2（ B2 R） and angiogenesis like protein-2（ Angptl 2） in T2 DM rats. Methods 60 healthy Sprague Dawley（ SD） rats were randomly divided into diabetic mellitus group（ n = 40） and normal control group（ n =20）. Then rats of diabetic group were fed with high calorie and high sugar diet for 4 weeks,following by one dose of streptozotocin（ STZ） via intraperitioneal injection. The model rats were randomly divided into the experimental group（ RYGB group,n = 15）（ 2 rats died after operation） and the diabetic mellitus sham operation group（ DM group,n = 15）. The rats of RYGB group were undertaken with RYGB,and the rats of DM group and NC group were undertaken sham operation. The level of weight,FBG,FINS,ISI,Angptl 2 and B2 R were detected before surgery and 4 weeks after surgery.Results Before the surgery,RYGB group compared with DM group,the level of weight,FBG,FINS,ISI,ANGPTL2 and B2 R were not statistically significant（ P〉0.05）. 4 weeks after the surgery in RYGB group,the level of weight,FBG and FINS were decreased（ P〈0. 05）,and ISI,ANGPTL2 and B2 R were gradually increased（ P〈0. 05）.However,in DM and NC group,there was no significant difference in the level of weight,FBG,FINS,ISI,ANGPTL2 and B2 R before and 4 weeks after surgery（ P〉0.05）.Conclusions Roux-en-y gastric bypass surgery may reduce weight and blood glucose by decreasing the level of B2 R and Angptl2 in type 2 diabetic rats.
作者
尹智冲
王跃生
张立海
尹彦斌
张久玲
杨德名
YIN Zhi- chong(department of general surgery, the first affiliated hospital of Jiamusi University, Jiamusi, Heilongjiang, 524000, Chin)
出处
《齐齐哈尔医学院学报》
2018年第5期499-502,共4页
Journal of Qiqihar Medical University
基金
佳木斯大学研究生创新基金项目(YM2016_007)
佳木斯大学科学技术校级面上项目(13Z12015140)
黑龙江省教育厅科研项目(2016-KYYWF-0595)
2016年黑龙江省卫生计生委科研项目(2016-313
2016-305)
关键词
2型糖尿病
胃旁路术
缓激肽受体2
血管生成样蛋白2
Type 2 diabetes mellitus
Roux- en- Y gastric bypass
Bradykinin receptor 2
Angiogenesis like protein-2
