摘要
目的 :观察迷走神经肝支及R oux-en-Y胃旁路术对2型糖尿病大鼠空腹血清及餐后30m in胰高血糖素样肽-1(G LP-1)的影响,并分析交互作用。方法:对55只雄性SD大鼠腹腔注射链脲佐菌素建立2型糖尿病模型。将建模成功的45只大鼠分成假手术组10只(S组);假手术+迷走神经肝支切断组11只(SV组);手术组12只(R YG B组);手术组+迷走神经肝支切断组12只(R YG B V组)。检测术前以及术后1、4、8周的空腹、50%葡萄糖溶液(2 g/kg)灌胃30 m in后血清G LP-1浓度,并分析术后1、4、8周R YG B和迷走神经肝支对血清G LP-1的影响。结果:4组大鼠术前、术后1、4、8周空腹血清G LP-1水平无变化(P>0.05)。4组大鼠术前餐后30 m in血清G LP-1水平无差异(P>0.05)。术后1周,R YG B明显提高餐后G LP-1水平,切断迷走神经肝支降低餐后G LP-1水平(S组21.57±2.40 pm ol/L,SV组14.48±1.62 pm ol/L;R YG B组43.07±2.76 pm ol/L;R YG B V组34.50±2.49 pm ol/L;P<0.05)。术后4周,R YG B明显提高餐后G LP-1水平,切断迷走神经肝支稍微降低餐后G LP-1水平(S组23.14±2.09pm ol/L;SV组19.16±2.42 pm ol/L;R YG B组57.33±2.33 pm ol/L;R YG B V组53.95±1.98 pm ol/L;P<0.05)。术后8周,R YG B维持较高餐后G LP-1水平,切断迷走神经肝支对G LP-1无影响(S组22.94±2.38 pm ol/L;SV组23.45±2.49 pm ol/L;R YG B组55.09±3.29 pm ol/L;R YG B V组55.21±4.02 pm ol/L;P>0.05)。R YG B对餐后血清G LP-1影响的主效应由术后1周时20.76 pm ol/L提高至术后8周时31.96 pm ol/L(P<0.05),迷走神经肝支的主效应由术后1周时8.05 pm ol/L降至术后8周时0.32 pm ol/L(P>0.05),不存在交互作用(P>0.05)。结论:就远期效应而言,行R YG B治疗2型糖尿病时,迷走神经肝支的保留与否并不影响血清G LP-1的水平。
Objective：To investigate the effects of the hepatic branch of vagus and Roux-en-Y gastric bypass on the level of fasting and postprandial serum glucagon-like peptide-1 （GLP-1） in type 2 diabetic rats,and analyze their interactions.Methods：In order to create the model of type 2 diabetes,55 male SD rats were injected with Streptozotocin intraperitoneally.After completion,45 rats were divided into four groups：sham operation （S,n=10）,sham operation within the hepatic branch of vagus to be cut off（SV,n=11）,Roux-en-Y gastric bypass（RYGB,n=12）,and RYGB without reserving the common hepatic branch （RYGBV,n=12）.Levels of fasting and postprandial serum GLP-1 within 50 percents of glucose solution （2 g/kg） after lavaging for 30 min were detected before operation and postoperative at 1,4,8 weeks.Meanwhile,Interactions between RYGB and the common hepatic branch were also calculated.Results：Levels of fasting serum GLP-1 didn＇t show any differences before and after operation （P＞0.05）,and preoperative postprandial serum GLP-1 among the four groups also had no significance （P＞0.05）.However,RYGB obviously improved postprandial GLP-1 levels after 1 week（P＜0.05）,and maintained a high standard no matter after 4 or 8 weeks（P＜0.05）.While it slightly reduced the concentration without reserving the hepatic branch of vagus after 1 week （P＜0.05）.When it comes to 8 weeks,cutting off or not has no effects on the level of glp-1 （P＞0.05）.Overall,the main effect of RYGB on postprandial serum GLP-1 had increased 20.76 pmol/L to 31.96 pmol/L.But,the impact of the common hepatic branch dropped from 8.05 pmol/L to 0.32 pmol/L.No interaction was found （P＞0.05）.Conclusion：Roux-en-Y gastric bypass surgery could obviously increase postprandial serum concentrations of glp-1,and maintained at a relatively high level.Nevertheless,preserving hepatic branch of the vagus only appropriately increase postprandial levels of GLP-1 at an early stage,and its main effect would gradually di
出处
《中国现代普通外科进展》
CAS
2015年第4期259-264,共6页
Chinese Journal of Current Advances in General Surgery
基金
国家自然科学基金项目(81270551)
上海市青年科学基金项目(12ZR1454300)
