期刊文献+

烟草半胱氨酸蛋白酶的生物学分析 被引量:2

Analysis on Biological of Cysteine Protease in Tobacco
下载PDF
导出
摘要 [目的]研究烟草半胱氨酸蛋白酶的生物学特性。[方法]通过PROTPARAM、CLUSTAL、MEGA、TMHMM、WOLF-PSORT、SOPMA工具对烟草半胱氨酸蛋白酶的理化特性、进化树和跨膜结构进行生物学分析。[结果]NtCP-3和NtCP-4氨基酸个数(361)最大,NtCP-5氨基酸个数(349)最小;NtCP-1、NtCP-2、NtCP-3和NtCP-4为酸性,NtCP-5为碱性;NtCP均属于稳定性蛋白,且NtCP-1和NtCP-2的耐热性较好;NtCP-3和Nt CP-4属于亲水性蛋白,其余为两性蛋白;NtCP被分为3个亚组,NtCP-1和NtCP-2为一组,NtCP-3和NtCP-4为一组,NtCP-5单独成一组;NtCP均属于跨膜蛋白,且NtCP-5具有2个跨膜结构。[结论]烟草半胱氨酸蛋白酶的生物学特性为广泛地参与烟草的生理反应奠定基础。 [Objective] The research aimed to study the biological characteristics of tobacco cysteine protease. [Method]The physical and chemical properties,phylogenetic tree and transmembrane structure of tobacco cysteine protease were analyzed by using PROTPARAM,CLUSTAL,MEGA,TMHMM,WOLF-PSORT and SOPMA respectively.[Result]The amino acid numbers of NtCP-3 and NtCP-4 were the largest( 361),and the NtCP-5 amino acid number was the smallest( 349); NtCP-1,NtCP-2,NtCP-3 and NtCP-4 were acidic,and NtCP-5 was alkaline; NtCP belonged to the stability protein,and the heat resistance of NtCP-1 and NtCP-2 were better; NtCP-3 and NtCP-4 were hydrophilic proteins,and the rest were amphoteric proteins; NtCP was divided into three subgroups,NtCP-1 and NtCP-2 were one group,NtCP-3 and NtCP-4 were a group,and NtCP-5 was a single group; NtCP belonged to transmembrane protein,and NtCP-5 had 2 transmembrane structures.[Conclusion]The biological characteristics of tobacco cysteine protease laid the foundation for extensively participating in the physiological response of tobacco.
作者 马世明 罗家佐 王德勋 户艳霞 范志勇 苏家恩 MA Shi-ming;LUO Jia-zuo;WANG De-xun(Nanjian County Branch of Dali Tobacco Company, Dali,Yunnan 675700;Weishan Branch of Dali Tobacco Company, Dali,Yunnan 672400;Dali Prefecture Tobacco Company, Dali,Yunnan 671000)
出处 《安徽农业科学》 CAS 2018年第16期17-19,共3页 Journal of Anhui Agricultural Sciences
基金 中国烟草总公司云南省公司项目(2015YN20 2016YN10)
关键词 烟草 半胱氨酸蛋白酶 生物学 Tobacco Cysteine protease Biology
  • 相关文献

参考文献4

二级参考文献79

共引文献57

同被引文献18

引证文献2

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部