摘要
为揭示茶树（Camellia sinensis）硒代半胱氨酸甲基转移酶（Selenocysteine methyltransferase，SMT）基因CsSMT的生物学功能及其分子调控机理，利用生物基因组学数据库和生物信息学分析软件，对CsSMT进行生物信息学分析，预测该基因编码产物的理化性质、结构与功能，同时构建CsSMT同源基因的系统进化树。结果表明：GsSMT基因cDNA全长1368bp，开放阅读框位于50-1105bp处。编码产物为稳定的亲水性蛋白，分子式为C1670H2645N459o532S15,无跨膜结构，无信号肽，定位于细胞质基质。二级结构以α-螺旋和无规则卷曲为主，三维建模成功。系统进化分析表明，CsSMT基因mRNA与拟南芥同型半胱氨酸S-甲基转移酶基因（Homocysteine S-methyhransferase，HMT）聚为一组。
To explore the biological function of tea plant （Camellia sinensis） selenocysteine methyltransferase （SMT） gene （CsSMT） and its molecular regulation mechanism, the physicochemical characteristics, structures and functions of CsSMT enzyme were predicted and analyzed with biological genome database and software tools. Meanwhile, the phylogenetic tree of CsSMT and its homologous genes was constructed. The result showed that CsSMT eDNA is 1 368 bp, its open reading frame （ORF） is located at 50 - 1105 bp. CsSMT enzyme located in the cytoplasmic matrix, molecular formula Ci670H2645N4590532S18. It is a stable hydrophilic protein without signal poptide as well as transmembrane domain. The secondary structure of CsSMT enzyme is primarily composed of alpha helia and random coil, and its 3D model is constructed. Phylogenetic analysis indicated that CsSMT enzyme and homocysteine S-methyltransferase （HMT） may have similar function, with the ability to methylate cysteine and homecysteine.
出处
《西南农业学报》
CSCD
北大核心
2013年第6期2221-2226,共6页
Southwest China Journal of Agricultural Sciences
基金
贵州省农业科学院研究生科研创新基金项目[黔农科合(创新基金)2011006号]
