摘要
甜菜碱高半胱氨酸甲基转移酶(BHMT)是一种甲基代谢酶,在动物和微生物体内存在,能催化甜菜碱向高半胱氨酸转移甲基,促进甲硫氨酸形成。本试验通过RT-PCR扩增猪肝脏BHMT基因ORF的cDNA序列,成功构建了p3301-BHMT植物表达载体,并利用农杆菌介导法转化玉米萌动胚,经PPT筛选和PCR检测,初步确定获得4株抗性植株。试验结果将为研究BHMT基因在植物中的作用,提高玉米甲硫氨酸含量和抗逆性提供试验材料。
Betaine-homocysteine methyl transferase（BHMT）,a methyl-metabolizing enzyme found in animals and micro-organisms,can catalyze methyl of betaine shifting to homocysteine and forming methionine.However,there has been no report of its expression in plant until now.This study amplified the ORF sequence of BHMT from Sus scrofa liver by RT-PCR,and successfully constructed the plant eukaryotic expression vector p3301-BHMT.The p3301-BHMT vector was transformed into germinating embryo of maize by Agrobacterium-mediated method.4 transgenic plantlet strains were obtained through screening by repeatedly spraying PPT and genomic DNA PCR with specific primers.The results provide useful materials for improving methionine content and stress resistance in maize with BHMT.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2010年第5期18-21,25,共5页
Journal of Hebei Agricultural University
基金
河北农业大学博士科研启动基金
关键词
BHMT
植物表达载体
玉米
转化
BHMT
plant expression vector
maize
transformation
