摘要
建立了一种绿色、快速、高效萃取和富集中药急性子中4种凤仙萜四醇皂苷的方法。采用离子液体并结合超声辅助萃取急性子中的化学成分,利用固相萃取小柱富集提取液中的凤仙萜四醇皂苷。通过考察离子液体种类及用量、p H值、超声时间、固相萃取剂和洗脱剂等实验条件对提取率的影响,确立了最优萃取剂为[C6mim]Br,最优萃取条件:萃取剂用量为0.9 m L,提取液p H=7.0,超声为30 min,最优富集条件:固相萃取小柱填料为0.9 g CHP20/P120,上样流速为1.5 m L/min,洗脱剂为1.5 m L甲醇。在最优条件下,4种凤仙萜四醇苷加标回收率在92.1%~108.2%之间,相关系数范围为0.9945~0.9975,检出限范围为1.8~4.5μg/m L,RSD<3.9%。本方法与传统回流提取方法提取效果接近,可准确测定急性子中4种凤仙萜四醇苷含量。
A green,rapid and efficient method was developed for the extraction of 4 kinds of hosenkosides from the seeds of Impatiens balsamina L. The hosenkosides were extracted by ionic liquid,and then enriched by solid phase extraction. The effects of the kinds and volume of ionic liquid,p H value,ultrasonic time,solid phase extractant and eluent on the extraction fields were investigated and optimized. Under the optimum conditions,the recoveries of 4 kinds of hosenkosides were between 92. 1% and 108. 2%,the correlation coefficients were 0.9945-0.9975,and the detection limits were 1.8-4.5 μg/m L. The RSD values were all less than 3.9%. The experimental results showed that this method was fast,efficient,environmental protection.This study provided a reference for the extraction and enrichment of trace components in Chinese medicinal herbs.
作者
吴谦
林花
刘迎
金永日
黎鹏
刘伟杰
李绪文
WU Qian1, LIN Hua2, LIU Ying1,JIN Yong-Ri1,LI Peng1, LIU Wei-Jie1, LI Xu-Wen1(College of ChemistryI, Clinical Laboratory of First Hospital2, Jilin University, Changchun 130021, Chin)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第6期910-916,共7页
Chinese Journal of Analytical Chemistry
关键词
离子液体
固相萃取
急性子
凤仙萜四醇皂苷
超声辅助
Ionic liquid
Solid phase extraction
Seeds of Impatiens balsamina L
Hosenkosides
Ultrasonicassisted
