摘要
目的:探讨热休克蛋白(HSP)70基因多态性与慢性心力衰竭(CHF)患者预后的相关性。方法:将408例CHF患者纳入研究,根据随访2年内是否发生心血管事件将患者分为事件组与非事件组,采用聚合酶链反应-限制性内切酶分析法检测HSP70-1基因+190G/C、HSP70-2基因+1267A/G和HSP70-hom基因+2437T/C多态性,并收集患者临床资料进行分组及统计学分析。结果:事件组与非事件组患者年龄(t=2.392,P=0.017),脑钠肽(BNP)水平(t=3.922,P=0.000),NYHA分级(χ~2=5.100,P=0.024),左室舒张末期内径(LVEDD)(t=2.872,P=0.004)、右室舒张末期内径(RVEDD)(t=2.232,P=0.026)、左室射血分数(LVEF)超声测值(t=4.338,P=0.000)等参数比较均差异有统计学意义。2组HSP70-2基因+1267A/G位点基因型和等位基因分布频率比较差异均有统计学意义(χ~2=8.480,P=0.014;χ~2=7.643,P=0.006),在隐性基因模型下HSP70-2基因+1267A/G位点基因多态性与CHF患者发生心血管事件相关(χ~2=9.877,P=0.002);HSP70-1基因+190G/C和HSP70-hom基因+2437T/C位点基因型和等位基因分布频率均差异无统计学意义(均P>0.05),且在显性基因模型和隐性基因模型下两位点基因多态性均与CHF患者发生心血管事件无关(均P>0.05)。Logistics多因素回归分析结果显示HSP70-2基因+1267A/G位点GG基因型是CHF患者发生心血管事件的危险因素(OR=1.362;95%CI 1.046~2.701;P=0.003)。结论:HSP70-2基因+1267A/G多态性与CHF患者预后相关,GG基因型可增加CHF患者发生心血管事件的风险。HSP70-1基因+190G/C和HSP70-hom基因+2437T/C多态性可能与CHF患者预后关联不大。
Objective：To investigate the genetic association between heat shock protein（HSP）70 gene polymorphism and prognosis in patients with chronic heart failure（CHF）.Method：The totals of 366 CHF patients from January 2013 to July 2015 were recruited in this study.According to whether with cardiovascular events,they were divided into the event group and the non-event group.The genotype was determined by polymerase chain reaction-restriction enzyme analysis for the polymorphism of the HSP70-1 gene＋190 G/C,HSP70-2 gene＋1267 A/G and HSP70-hom gene ＋2437 T/C.The clinical characteristics and the incidence of cardiovascular event were compared between two groups.Result：There was significant difference in age（t=2.392,P=0.017）,level of serum BNP（t=3.922,P=0.000）,NYHA classification（χ^2=5.100,P=0.024）,left ventricular end diastolic diameter（t=2.872,P=0.004）,right ventricular end diastolic diameter（t=2.232,P=0.026）and 1 eft ventricular ejection fraction（t=4.338,P=0.000）between the event group and the non-event group.There were significant difference in the genotype and allele frequencies of HSP70-2 gene ＋1267 A/G between the event group and the non-event group（χ^2=8.480,P=0.014;χ^2=7.643,P=0.006）.And significant association could be found in recessive gene model between HSP70-2 gene＋1267 A/G polymorphism and cardiovascular events（χ^2=9.877,P=0.002）.However,genotype and allele frequencies of HSP70-1 gene ＋190 G/C and HSP70-hom gene ＋2437 T/C did not differ significantly between two groups（all P〉0.05）.There was no significant association between HSP70-1 gene＋190 G/C and HSP70-hom gene ＋2437 T/C polymorphism and cardiovascular events under the dominant gene model and recessive gene model（all P〉0.05）.Multivariate Logistic regression analysis showed that GG genotype of HSP70-2 gene＋1267 A/G was risk factor of cardiovascular events for CHF patients（OR=1.362;95%CI1.046~2.701;P=0.003）.Conclusion：Polymorphism of the HSP70-2 gene＋1267 A/G may associate
作者
刘锋瑞
高红艳
王丽
刘亚娟
张伟
王建军
LIU Fengrui;GAO Hongyan;WANG Li;LIU Yajuan;ZHANG Wei;WANG Jianjun(Department of Emergency, First Affiliated Hospital of Xi＇an Medical College, Shaanxi, xi＇an, 710077,China;Department of Medicine, Xi＇an North Car Hospital;Department of Medicine, Dongguan Branch of Affiliated Hospital of Yan＇an University)
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2018年第6期572-577,共6页
Journal of Clinical Cardiology
关键词
慢性心力衰竭
热休克蛋白70
单核苷酸多态性
预后
chronic heart failure
heat shock protein 70
single nucleotide polymorphism
prognosis
