摘要
目的:观察电针肺与大肠俞募配穴联合大承气汤对急性胰腺炎(AP)大鼠肺和肠道炎性反应损伤的影响,探讨电针与大承气汤协同增效的机制。方法:SD大鼠随机分为假手术组、模型组、大承气汤组、电针治疗组和针药合用组,每组8只。采用改良3%牛磺胆酸钠(0.1mL/100g)逆行胰胆管注射法复制AP模型。电针治疗组和针药合用组电针双侧"肺俞"与"大肠俞""中府""天枢"各3次,每次20min,每次间隔7h。造模24h后,针药合用组和大承气汤组给予大承气汤灌胃1次。采用双抗夹心ELISA法测定血清白细胞介素-6(IL-6)、IL-10含量和肺、大肠组织髓过氧化物酶(MPO)、丙二醛(MDA)含量,HE染色观察肺、大肠、胰腺组织病理变化并予以病理评分。结果:与假手术组比较,模型组胰腺、肺、大肠病理评分,肺与大肠MDA、MPO含量,血清IL-6、IL-10含量均明显升高(P<0.05);与模型组比较,大承气汤组、电针治疗组、针药合用组胰腺、肺、大肠病理评分,肺与大肠MDA、MPO含量,血清IL-6含量明显降低(P<0.05),血清IL-10含量升高(P<0.05)。与大承气汤组和电针治疗组比较,针药合用组肺与大肠病理评分、MPO和MDA含量,血清IL-6含量明显降低(P<0.05),血清IL-10含量升高(P<0.05)。结论:肺与大肠俞募配穴法电针与大承气汤针药合用与单纯大承气汤、电针相比,在调控急性胰腺炎大鼠氧化应激水平和炎性反应中更有优势,以协同减轻肺肠损伤。
Objective To explore the effectiveness of electroacupuncture （EA） stimulation of Shu-and Mu-points of Lung and Large Intestine Meridians combined with administration of Da-Cheng-Qi Decoction （OCQD） in the treatment of inflammatory injury in rats with acute pancreatitis, so as to reveal their synergetic anti-inflammatory effect. Methods Forty male SD rats were randomly divided into five groups： normal control, model, DCQD, EA, EA＋ DCQD, with 8 rats in each group. All the rats except those in the normal group received a retrograde biliopancreatic duct injection of sodium Taurocholate （3.0%, 0. 1 mL/ 100 g） to induce an acute pancreatitis model. Twenty-four hours after modeling, EA （4 Hz/50 Hz） was applied to bilateral Shupoints ＂Feishu＂ （BL 13） and ＂Dachangshu＂ （BL 25）, and Mu-points ＂Zhongfu＂（LU 1 ） and ＂Tianshu＂ （ST 25） for 20 min, once every 7 h,3 times in total. Rats of the DCQD and EA＋ DCQD groups were given intra-gastric gavage of DCQD （ 1 mL/100 g） 24 h after modeling, and those of the other 3 groups were given intra-gastric gavage of same dosage of normal saline. The histopathological changes of the pancreas, lung and large intestine tissues were observed after H. E. staining and scored according to Schmidt＇s and colleagues＇ methods （the severity of edema, inflammation, hemorrhage, necrosis）. The concentrations of interleu- kin-6 （IL-6） and IL-10 in the serum, and those of myeloperoxidase （MPO） and malonaldehyde （MDA） in the lung and large intestine tissues were detected by using double-antibody sandwich ELISA. Results After modeling, the histopathological scores of oancreas, luna and larae intestine tissues,the concentrations of MPO and MDA in lung and larqe intestine, and the levels of IL-6 and IL-10 in serum were significantly increased in the model group compared with the normal control group （P〈0.05）. Following the treatment, the histopathological scores of pancreas, lung and large intestine tissues, the contents of MPO
作者
陈欢
苏杭
袁玲
苗艺凡
张玉梅
李娟
唐文富
CHEN Huan;SU Hang;YUAN Ling;MIAO Yi-fan;ZHANG Yu-mei;LI Juan;TANG Wen-fu(Department of Integrative Medicine, West China Hospital of Sichuan University, Chengdu 610041, China)
出处
《针刺研究》
CAS
CSCD
北大核心
2018年第6期353-359,共7页
Acupuncture Research
基金
国家自然科学基金项目(No.81374042
81573857)
关键词
急性胰腺炎
电针
大承气汤
俞募配穴
炎性反应
Acute pancreatitis
Electroacupuncture
Da-Oheng-Qi Decoction
Shu- and Mu-points
Inflammatory response
