摘要
目的以内切-1,3-β-葡聚糖酶的最佳酶解条件对不同种质资源蒙古黄芪(移栽和野生)和膜荚黄芪(移栽和野生)多糖进行酶解,通过荧光辅助糖电泳(FACE)获得糖指纹图谱,用于黄芪的种质资源鉴别和评价。方法利用SMICA软件对数据进行主成分分析及t检验,获得区分不同种质资源黄芪的差异性糖组分。结果内切-1,3-β-葡聚糖酶酶解产物中五糖和六糖可以作为区分野生蒙古黄芪和野生膜荚黄芪的差异性糖片段,三糖、四糖和五糖可以作为区分移栽蒙古黄芪与移栽膜荚黄芪的差异性糖片段,五糖和六糖可以作为区分膜荚黄芪(野生和移栽)的差异性糖片段;结论内切-1,3-β-葡聚糖酶降解的多糖产物能很好地鉴别黄芪种属和生长方式。为黄芪药材的品质评价及活性寡糖的筛选奠定了基础。
Objective Enzymatic hydrolysis of Astragali Radix polysaccharides from different germplasm resources Astragalusmembranaceus var. mongholicus（MG）（cultured and natural） or Astragalusmembranaceus（MJ）（cultured and natural） was carried out by the best enzymolysis conditions of endo-1,3-β-glucanase. Saccharide fingerprints were obtained for the identification and evaluation of the germplasm resources of Astragali Radix by Fluorophore-assisted Carbohydrate Electrophoresis（FACE）. Methods The data were analyzed by principal component analysis and t test using SMICA software to distinguishdifferential sugar segments among different germplasm resources of Astragali Radix. Results Pentasaccharide and hexasaccharide of endo-1,3-β-glucanasehydrolyzate could be used as differentiated saccharide fragments between natural MG and MJ.Trisaccharide, tetrasaccharide, and pentasaccharide could be used as differentiated saccharide fragments to distinguish the cultured MG and MJ.The pentasaccharide and hexasaccharide can be used as differential fragments to distinguish MJ（culturedandnatural）. Conclusion Thepolysaccharide products degraded by endo-1,3-β-glucanase can well distinguish Astragali Radix species（MG andgrowth mode（cultured and natural Astragali Radix）. This study laid the foundation for the quality evaluation of Astragali Radix and screening of active oligosaccharides.
作者
王迪
刘磊
李科
李爱平
刘月涛
秦雪梅
杜冠华
张翔
WANG Di;LIU Lei;LI Ke;LI Ai-ping;LIU Yue-tao;QIN Xue-mei;DU Guan-hua;ZHANG Xiang(Modem Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;Institute of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100050, China;University of Louisville, Louisville 40292, America)
出处
《中草药》
CAS
CSCD
北大核心
2018年第10期2320-2327,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(31200278)
教育部博士点新教师类基金项目(20131401120006)
山西省优秀人才科技创新项目(201705D211020)
山西省中药产业重点科技攻关项目(201603D311101)
国家中药标准化项目(ZYBZH-Y-JIN-34)
山西省科技攻关项目(2014ZD0401)
关键词
内切-1
3-β-葡聚糖酶
黄芪
多糖
指纹图谱
种质资源
主成分分析
endo-1,3-β-glucanase
Astragali Radix
polysaccharide
fingerprint
germplasm resources
principal component analysis
