摘要
目的:为了提高核桃粕利用价值,阐明核桃多肽抗氧化活性及其构效关系。方法:采用DA 201-C大孔树脂和Sephadex G-15葡聚糖凝胶分离纯化核桃粕蛋白酶解液,分析比较不同疏水性和不同分子量核桃多肽抗氧化活性及氨基酸组成特性。结果:经DA 201-C大孔树脂分离的多肽组分Ⅰ、Ⅱ、Ⅲ的抗氧化活性随洗脱溶剂疏水性增大而增大。组分Ⅲ经Sephadex G-15分离纯化得到a、b、c、d四个不同分子量多肽组分。分子量分布在400～700 Da的组分c具有最高的羟自由基清除能力,并含有较高的谷氨酸、天冬氨酸、亮氨酸、苯丙氨酸,与组分Ⅲ在氨基酸组成上具有相似性。结论:具有较好抗氧化活性的核桃多肽分子量小于800 Da,并具有疏水性较强,谷氨酸、天冬氨酸、亮氨酸、苯丙氨酸组成含量相对较高的结构特性。
Objective：In order to improve the utilization value of walnut dregs and elucidate the antioxidant activity of walnut peptides and its structure-activity relationship.Methods： DA 201- C macroporous resin and Sephadex G-15 glucan gel were used to isolate and purify the enzymatic hydrolysis of walnut dregs protein by complex protease. The antioxidant activity and amino acid composition of walnut peptides with different hydrophobicities and molecular weights were analyzed and composed. Results：The antioxidant activities of polypeptide fractions Ⅰ、ⅡandⅢ,which were separated by DA 201-C macroporous resin, increased with the increasing of the eluent hydrophobicity.Four different molecular weight polypeptide components of a, b, c, d were isolated and purified from component Ⅲ by Sephadex G-15.Component c, which was similar to component Ⅲ in amino acid composition, had the highest hydroxyl radical scavenging capacity and contained higher glutamate, aspartate, leucine and phenylalanine. Its molecular weight was distributed in 400 - 700 Da. Conclusion ： Walnut polypeptide with better antioxidant activity had a molecular weight of less than 800 Da, it had the characteristics of strong hydrophobicity and relatively high composition contents of glutamic acid, aspartic acid, leucine and phenylalanine.
作者
李汉洋
李建杰
王帅
徐兴华
蓝文言
梁宇鹏
张珍兰
荣瑞芬
LI Han-yang;LI Jian-jie;WANG Shuai;XU Xing-hua;LAN Wen-yan LIANG Yu-peng;ZHANG Zhen-lan;RONG Rui-fen(Department of Food Science, Beijing Union University, Beijing 100191, China;School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China;The Laboratory of Marine Glycodrug Research and Development, Marine Biomedical Research Institute of Qingdao, Qingdao 266100, China, 4.Walnut Industrial Technology Research Institute of Hebei（Xingtai） , Lincheng 054300, China, 5. Beijing Guang- sheng New Agricultural Technology Co., Ltd., Beijing 100193, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第13期1-7,13,共8页
Science and Technology of Food Industry
基金
北京市教育委员会科技发展计划面上项目(KM200811417010).
关键词
核桃多肽
抗氧化活性
分子量
氨基酸组成
walnut polypeptide
antioxidant activity
molecular weight
amino acid composition
