摘要
本文改进了法夫酵母虾青素高效液相色谱-紫外法(HPLC-UV)分析的样品前处理方法,减轻微孔滤膜吸附对HPLC分析法夫酵母虾青素的影响。研究了样品前处理过程中,滤膜材料、滤膜孔径及甲醇中二甲基亚砜(DMSO)含量对虾青素吸附的影响。结果表明:PES和Nylon滤膜对虾青素具有显著吸附作用,但滤膜材料及孔径对虾青素吸附作用的差异不显著;在甲醇中添加DMSO可显著减少滤膜对虾青素的吸附,当DMSO为7.5%时虾青素吸附量最小,加标回收率在95.11%~100.76%之间,日内、日间精密度(RSD)均小于2%。该方法快速、准确,可用于相关产品中虾青素含量测定。
Microfiltration of samples is a common operation in high performance liquid chromatography analysis,which has been found to be able to adsorb noticeable astaxanthin in our previous studies.The aim of this study was to improve sample preparation to reduce the effect of microfilter absorption on astaxanthin analysis.In this study,the effects of microfilter materials,pore size and the content of dimethyl sulfoxidein（DMSO）on the microfilter absorption of astaxanthin were investigated.The results showed that both polyethersulfone（PES）and Nylon microfilter could obviously adsorb astaxanthin,but filter materials and pore sizes had no detectable effects on astaxanthin absorption;the addition of DMSO into methanol could significantly reduce the adsorption of astaxanthin.When the content of DMSO was7.5%,the amount of absorbed astaxanthin was the least.In this case,the regression equation of astaxanthin calibration curve was Y=861648 X＋1992.5,the recoveries was within 95.11%-100.76%,and the intraday precision and interday precision were both less than 2%.Using the new method,a simple procedure was developed to extract and analyze astaxanthin in Phaffia rhodozyma.In short,the study helps to improve the sensitivity,reliability,accuracy and reproducibility of astaxanthin analysis and develop the procedure for the extraction and analysis of astaxanthin in P.rhodozyma,providing a technological reference for the simple,convenient and accurate quantitation of astaxanthin in biological materials and related products.
作者
王春
杜希萍
姜泽东
黄高凌
肖安风
倪辉
WANG Chun;DU Xi-ping;JIANG Ze-dong;HUANG Gao-ling;XIAO An-feng;NI Hui(College of Food and Bioengineering ,Jimei University ,Xiamen,Fujian 361021;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen,Fujian 361021;Research Center of Food Biotechnology of Xiamen City ,Xiamen,Fujian 361021)
出处
《分析科学学报》
CAS
CSCD
北大核心
2018年第3期352-356,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.31501448)
福建省教育厅项目(No.JA15266)
