摘要
以多糖提取率为评价指标,水提醇沉法,单因素实验基础上,响应面优化苦胆草多糖的最佳提取工艺,并测定其抗氧化活性。确定苦胆草多糖的最佳提取工艺为料液比1∶40(g/mL),提取温度65℃,提取时间7.5 h,多糖提取率为3.48±0.96%。苦胆草多糖对DPPH·自由基最大清除率为24.01%,表明其有一定的抗氧化活性。
Taking the yield of polysaccharide as indicator, using single factor test, response surface methodology （RSM） was employed to optimize polysaccharides of andrographis paniculata by water extracting-alcohol precipitating method. The result showed that the best extraction process of polysaccharides of andrographis paniculata were ： the ratio of material to liquid 1 ： 40 （g/mL）, at 65 ℃ and extracting for 7.5 h. The yield of polysaccharide was 3.48 ± 0.96%. The maximum DPPH · scavenging rate of andrographis paniculata polysaccharides of 24.01% shows that it has certain antioxidant activity.
作者
李月
徐兵
范媛媛
王昀
左绍远
陈贵元
LI Yue;XU Bing;FANG Yuan- yuan;WANG Yun;ZUO Shao-yuan;CHEN Gui-yuan(College of Basic Medicine, Dali University, Dali 671000;Provincial Key Laboratory of Entomology Biopharmaceutical R＆D, Dali 671000;Fuling Central Hospital of Chongqing City, Chongqing 408000)
出处
《中国食品添加剂》
CAS
2018年第5期62-68,共7页
China Food Additives
基金
大理大学博士科研启动费项目(KY83701401)
云南省教育厅科学研究基金项目(2015Z147)
云南省昆虫生物医药研发重点实验室项目(2015)
关键词
苦胆草
多糖
响应面法
抗氧化活性
andrographis paniculata
polysacchaddes
response surface method
antioxidant activity
