摘要
目的研究不同运动强度对糖尿病大鼠心肌纤维化的影响。方法高脂饲养加小剂量链脲佐菌素建2型糖尿病大鼠模型，分低、中、高运动量组、糖尿病模型组；另设标准饲料饲养的正常对照组，每组6只。采用颈总动脉插管、Masson染色和免疫组织化学等方法检测大鼠动脉血压、心肌形态学改变和纤维化指标。结果与正常大鼠相比，糖尿病大鼠体重、血糖和血压均升高，心肌细胞排列紊乱，胶原面积百分比增加，Ⅰ型和Ⅲ型胶原表达增加（P〈0．05），心肌出现明显纤维化。给予不同强度的运动，上述指标均有所改善，但只有中运动量组改善效果明显，差异有统计学差异（P〈0．05）。结论中度运动可以明显减轻糖尿病大鼠心肌纤维化程度，改善心功能。
Objective To investigate the effects of exercise intensity on myocardial fibrosis in diabetic rats. Methods High - fat diet and a low dose of streptozotocin were used to induce type 2 diabetes mellitus （T2DM） in rats. The resultant diabetic rats were divided into a T2DM group, and T2DM ＋ low - intensity exercise （ DM ＋ LM ）, T2DM ＋ moderate - intensity exercise （ DM ＋ MM） and T2DM ＋ high - intensity exercise （ DM ＋ HM） groups. Meanwhile, normal di- et was provided for normal rats which were selected as the control Then, blood pressure was measured by carotid artery intubation. HE, Masson and immunohistochemical staining were used to observe pathological characteristics of the hearts. Results Compared with normal rats, diabetic rats showed increased body weight, fasting blood glucose and blood pressure, irregular collagen structure network in the interstitial and perivascular area of cardiac muscles, and increased collagen area percentage and types Ⅰ and Ⅲ collagen expression （P 〈 0.05 ）. These indexes were improved after different in- tensity of exercises, where remarkable improvement was found in the DM ＋ MM group （ P 〈 0.05 ）. Conclusions Moderate intensity exercise can markedly relieve cardiac fibrosis in diabetes rats and improve cardiac function.
作者
尚文康
马艳红
陈继磊
李翠
乔伟丽
SHANG Wenkang;MA Yanhong;CHEN Jilei;LI Cui;QIAO Weili(Department of Physiology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China)
出处
《徐州医科大学学报》
CAS
2018年第5期302-306,共5页
Journal of Xuzhou Medical University
基金
国家自然科学基金（81500177）
关键词
运动
糖尿病
心肌纤维化
exercise
diabetes mellitus
cardiac fibrosis
