摘要
目的确定可能导致膀胱癌的mi RNAs,并研究其在姜黄素抗癌作用中的机制。方法对T24和SV-HUC-1细胞进行了芯片测序和q PCR分析。检测mi R-7641在含姜黄素或不含姜黄素的T24和SVHUC-1细胞株中的潜在致瘤性。并使用蛋白质印迹分析p16是mi R-7641在T24细胞中作用的重要靶基因。结果姜黄素诱导mi R-7641的表达下调及p16的上调,而p16是mi R-7641的转录后水平靶点之一,从而导致膀胱癌细胞凋亡增加。结论研究表明姜黄素可能是治疗无肌肉浸润性膀胱癌的临床治疗的潜在有效药物。
Objective To identify the dys-regulated miRNAs in bladder cancer and examine their role in the anticancer effects of curcumin. Methods A microarray profiling and qPCR analysis were conducted in the T24 and SV-HUC-1 cell lines. We examined the potential tumorigenicity of miR-7641 in the T24 and SVHUC-1 cell lines with or without curcumin. Western blot analysis showed that p16 is a target of miR-7641 in T24 cells. Results Curcumin induces the downregulation of miR-7641 and subsequent upregulation of p16 which is a target of miR-7641 at the post-transcriptional level, which leads to the decreased invasion and increased apoptosis of bladder cancer cells. Conclusion The study shows that curcumin may be a candidate agent for the clinical management of non-muscle-invasive bladder cancer.
作者
吴雅莉
鲁琼
王洋波
徐冉
原海燕
屈健
周艳钢
唐甜甜
WU Ya-li;LU Qiong;WANG Yang-bo;XU Ran;YUAN Hai-yan;QU Jian;ZHOU Yan-gang;TANG Tian- tian(Department of Science and Education, Hunan Provincial Maternal and Child Health Care hospital, Changsha 410008;Department of Pharmacy, Second Xiangya Hospital, Central South University, Changsha 41 O011;Department of Urology, Second Xiangya Hospital, Central South University, Changsha 410011)
出处
《中南药学》
CAS
2018年第5期641-645,共5页
Central South Pharmacy
基金
湖南省自然科学基金(项目编号:2016JJ2072)
