摘要
活性氧自由基（ROS）是细胞有氧代谢过程中产生的一类活性物,利用食源蛋白制备具有抗氧化能力的天然活性肽是当前的研究热点。本研究以机械冷榨芝麻粕为原料,比较了解淀粉芽孢杆菌、植物乳杆菌单菌及其混菌发酵芝麻粕制备芝麻多肽的过程,以DPPH·与·OH清除能力为评价指标,研究其体外抗氧化活性。数据表明,解淀粉芽孢杆菌、植物乳杆菌单菌及其混菌发酵芝麻粕36 h,芝麻多肽提取液中芝麻多肽的浓度分别达到9.841 mg/m L、11.620 mg/m L、14.412 mg/m L,DPPH·清除率分别是71.378%、77.013%、87.622%,·OH清除率分别是68.427%、82.507%、92.460%。因此,芝麻多肽具有较强的抗氧化活性,芝麻粕是制备抗氧化活性肽的良好原料;微生物发酵制备芝麻多肽时,解淀粉芽孢杆菌与植物乳杆菌混菌发酵显著优于单菌发酵,其多肽产量及DPPH·与·OH清除能力均优于相应单菌发酵。
Reactive Oxygen Species（ROS） produced in the process of cell aerobic metabolism is a kind of active species.The preparation of natural active peptides with antioxidant ability from food protein is a researching hotspot currently.In this research,the mechanically cold pressed sesame meal was used as raw material.We compared the preparation of sesame polypeptide from fermentation of sesame meal with Bacillus amyloliquefaciens,Lactobacillus plantarum subsp.plantarum and the mixture of above.DPPH · and ·OH scavenging ability was taken as evaluation index to study the antioxidant activity in vitro.The results showed that,after the fermentation of sesame meal with Bacillus amyloliquefaciens,Lactobacillus plantarum subsp.Plantarum and the mixed bacteria for 36 h,the concentrations of sesame polypeptide in the extracts were 9.841 mg/m L,11.620 mg/m L,14.412 mg/m L,respectively,the DPPH· scavenging ability were 71.378%,77.013%,87.622%,and the ·OH scavenging ability were 68.427%,82.507%,92.460%,accordingly.Therefore,sesame polypeptide showed high antioxidant activity,and sesame meal was a good raw material for the preparation of antioxidant active peptides.During the the preparation of sesame polypeptide by the fermentation of microorganism,there were more sesame polypeptides with the higher DPPH·and·OH scavenging ability could be extracted from the mixed fermentation of Bacillus amyloliquefaciens and Lactobacillus plantarum subsp.Plantarum than the corresponding single strain.
作者
袁艳超
贾泽
赖爱兰
王智玮
黄运红
龙中儿
Yuan Yanchao;Jia Ze;Lai Ailan;Wang Zhiwei;Huang Yunhong;Long Zhonger(College of Life Science, Jiangxi Normal University, Nanchang, 33002)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第5期2068-2073,共6页
Genomics and Applied Biology
基金
江西省农副产品绿色高值化利用创新团队项目(赣科发计字[2015]84号)资助
关键词
植物乳杆菌
解淀粉芽孢杆菌
混菌发酵
芝麻多肽
抗氧化活性
Lactobacillus plantarum subsp. Plantarum
Bacillus amyloliquefaciens
Mixed bacteria fermentation
Sesame polypeptide
Antioxidant activity
