摘要
该文采用高糖诱导建立果蝇代谢紊乱模型,研究丹参茎叶对该模型的作用及机制。检测果蝇体内葡萄糖、三酰甘油、蛋白质的含量,记录成蛹时间,基于代谢组学评价代谢紊乱模型的可靠性和丹参茎叶作用的机制。结果显示,与对照组（N组）相比,模型组（M组）果蝇体内葡萄糖、三酰甘油含量显著上升（P〈0.05）;与模型组相比,达美康阳性药物组（GLZ组）、丹参茎叶提取物中剂量组（SM-M组）和高剂量组（SM-H组）果蝇体内的葡萄糖含量显著降低（P〈0.05,P〈0.01）,GLZ组、丹参茎叶提取物高剂量组（SM-H组）果蝇体内的三酰甘油含量显著下降（P〈0.05,P〈0.01）。对代谢物轮廓主成分分析（PCA）和偏最小二乘法判别分析（PLS-DA）发现,经丹参茎叶提取物干预后,模型果蝇的代谢水平有所回调,通过差异代谢物筛选、文献比对和数据库检索,分析得到17个标志性化合物和4个主要代谢通路。丹参茎叶提取物在果蝇体内可能是通过影响组氨酸代谢、甘油磷脂代谢、戊糖和葡萄糖醛酸盐相互作用、甘油酯代谢等代谢通路发挥调节糖脂代谢的功效。该研究表明,丹参茎叶提取物能有效调节果蝇代谢紊乱模型的糖脂代谢恢复正常,为丹参茎叶资源价值发现和资源化利用提供参考。
To evaluate the effect and mechanism of aerial parts of Salvia miltiorrhiza（ SM） on high sugar-induced Drosophila melanogaster metabolic disorder model. The levels of glucose,triglyceride and protein in SM were detected; nymphosis time was recorded,and the reliability of metabolic disorder model as well as the mechanism of aerial parts of SM were evaluated based on metabonomics.The results showed that the levels of glucose and triglyceride in model group were significantly higher than those in normal control group（ P 〈 0. 05）. As compared with the model group,the glucose level was significantly decreased in gliclazide（ GLZ） group,SM medium（ SM-M） and high（ SM-H） dose groups（ P 〈 0. 05,P 〈 0. 01）; the triglyceride level was significantly decreased in GLZ group and SMH group（ P 〈 0. 05,P 〈 0. 01）. By principal component analysis（ PCA） and partial least squares discriminant analysis（ PLS-DA）,the metabolic level of model ones was recovered to a certain degree after intervention by aerial parts of SM. Seventeen marker compounds and four major metabolic pathways were obtained by screening differential metabolites,comparing literature and retrieving the database.The aerial parts of SM may regulate glycolipid metabolism through the impact on histidine metabolism,glycerophospholipid metabolism,pentose and glucuronate interconversions,cysteine and methionine metabolism and glycerolipid metabolism. Extract from aerial parts of SM can regulate the glycolipid metabolism of D. melanogaster metabolic disorder model and make it return to normal condition. This paper provides reference for the value discovery and resource utilization of the aerial parts of S. miltiorrhiza.
作者
俞辰亚代
于金高
顾俊菲
宿树兰
华永庆
段金廒
YU Chen-yadai;YU Jin-gao;GU Jun-fei;SU Shu-lan;HUA Yong-qing;DUAN Jin-ao(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanfing University of Chinese Medicine, Nanjing 210023, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第7期1484-1491,共8页
China Journal of Chinese Materia Medica
基金
江苏省中药资源产业化过程协同创新中心开放项目(ZDXM-2-7)
关键词
丹参茎叶
果蝇
糖脂代谢
资源价值发现
aerial parts of Salvia miltiorrhiza
Drosophila melanogaster
glucolipid metabolism
discovery of resource value
