摘要
以来源于一体式部分亚硝化-厌氧氨氧化(combined partial nitrification-anammox,CPNA)序批式反应器(sequencing batch reactor,SBR)工艺的活性污泥为对象,通过厌氧批式实验,比较3种添加剂(Fe^(2+)、氧化石墨烯、Fe^(3+))投加后对厌氧氨氧化菌的脱氮效果、活性、关键功能基因数量、群落结构和功能丰度的影响。实验结果表明:经过42 d的厌氧培养后,3种添加剂对厌氧氨氧化脱氮性能都有一定的强化作用,投加氧化石墨烯提高效果最好,它的总氮去除率最高能达到76.01%,平均总氮去除率高于对照组22.71%;而投加Fe^(2+)和Fe^(3+)组的平均总氮去除率分别仅高于对照组5.69%和1.72%。投加3种添加剂均能提高厌氧氨氧化菌活性,其中氧化石墨烯组的活性比对照组提高8.08%,Fe^(3+)组的活性比对照组提高10.02%。投加氧化石墨烯对厌氧氨氧化菌代谢过程中hzo基因数量的提高效果最好,比对照组提高6.83倍,投加Fe^(2+)和Fe^(3+)分别比对照组提高5.14倍和4.90倍;且投加氧化石墨烯对浮霉菌门富集扩培效果最好,其丰度比对照组提高44.51%,而投加Fe^(2+)和Fe^(3+)后丰度结果分别比对照组提高1.03%和稍低于对照组。投加氧化石墨烯对微生物代谢功能的提升效果最佳。
The activated sludge in the combined partial nitritation-anammox（CPNA） process in sequencing batch reactor（SBR） was selected, and influences on nitrogen removal efficiency, activity and hzo gene copies of anammox bacteria, microbial community structure and functional abundance were compared by respectively dosing ferrous ion, graphene oxides（GOs） and trivalent iron ion through anaerobic batch test. The results showed that after 42 days of anaerobic culture, the three additives enhanced the anammox nitrogen removal to some degree, among which the dosing GOs was the best. The highest nitrogen removal efficiency of dosing GOs was up to 76.01%, 22.71%higher than the control group, compared with just 5.69% and 1.72% higher than the control group by the addition of Fe^（2＋） and Fe^（3＋）, respectively. The activity of anammox bacteria was improved by dosing these three additives, 8.08%and 10.02% higher than the control group by dosing GOs and Fe^（3＋）, respectively. The copies of hzo gene which encodes the key enzyme in the process of anammox metabolism was the highest in the dosing GOs group, which was higher than the control group by 6.83 times, and those of addition of Fe^（2＋） and Fe^（3＋） were 5.14 times and 4.90 times higher than the control group, respectively. In addition, the addition of GOs was the best for the enrichment of Planctomycetes, which abundance was 44.51% higher than the control group, but those of addition of Fe^（2＋） and Fe^（3＋） were only 1.03% higher than the control group and slightly lower than the control group, respectively. The addition of GOs was the best for the enhancement of microbial metabolic function.
作者
姚丽
张俊亚
王红艳
王元月
魏源送
YAO Li;ZHANG Junya;WANG Hongyan;WANG Yuanyue;WEI Yuansong(School of Life Science, University of Science and Technology of China, Hefei 230026, China;State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;Department of Water Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330029, China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2018年第5期1490-1500,共11页
Chinese Journal of Environmental Engineering
基金
国家重点研发计划课题(2016YFD0501405)
国家水体污染控制与治理科技重大专项(2013ZX07312-001
2015ZX07203-005
2017ZX07102)
国家自然科学基金资助项目(21607167)
江西省科技计划项目(20151BBG70006)
关键词
厌氧氨氧化菌
添加剂
总氮去除率
厌氧氨氧化菌活性
微生物群落结构
anammox bacteria
additives
total nitrogen removal efficiency
anammox bacterial activity
microbialcommunity structure
