摘要
以核桃树叶为原料,采用微波辅助纤维素酶提取单宁,并用响应面法对提取工艺进行优化。结果表明:微波处理时间10 min、微波功率250 W、缓冲液pH值为4.0、酶解温度60℃、酶解时间100 min及酶液用量1.1%(体积分数)时,单宁的得率可达6.08%,高于无微波辅助所提取的单宁量。红外光谱分析显示:提取物中含有无色花翠素-3-葡萄糖苷分子结构类物质;抗氧化能力检测表明:微波联合纤维素酶法能够提高单宁对·OH、DPPH·和ABTS^+·等自由基的清除能力,其IC_(50)值均低于水浴加热法及超临界提取法。采用本方法提取的核桃树叶单宁不仅具有较高的得率,还具有较好的抗氧化性。
Tannins was extracted from Walnut Leaf with microwave-assisted cellulose enzyme method,and theexperimental result of response surface methodology （RSM）was employed to optimize the extraction process.The results showed that the extraction yield could reach 6.08%,under the following optimized experimentalconditions：1.1% （volume fraction）enzyme dosage,60 益 enzyme extraction temperature,100 min enzymeextraction time,4.0 pH value of buffer solution,and the sample treated 10 min with 250 W microwave power,which was higher than the sample obtained without microwave-assisted. Infrared spectrum analysis indicated theextraction contained many proanthocyanidin-like substances with 3-O-gallate acyl structure,and antioxidationcapacity assay showed that the microwave -assisted enzyme method could improve the scavenging activity oftannins on radicals,including· OH,DPPH·,and ABTS＋·. The half maximal inhibitory concentration values（IC50）were reduced respectively,compared with the water-bath heating and supercritical fluid method. Theextraction method in this paper not only could get higher yield of walnut leaf tannins,but also had betterantioxidation capacity.
作者
刘凤云
谢金枝
LIU Feng-yun;XIE Jin-zhi(Jiaozuo Adult Medical College, Jiaozuo 454000, Henan, Chin)
出处
《食品研究与开发》
CAS
北大核心
2018年第11期37-42,共6页
Food Research and Development
基金
湖南省科技厅科技计划项目(2016GK6185)
关键词
响应面优化
微波辅助提取
酶法
单宁
抗氧化能力
response surface methodology （RSM）optimization
microwave assisted extraction
enzyme method
tannin
antioxidation capacity
