摘要
目的考察益气复生方对脂多糖(lipopolysaccharide,LPS)诱导的胃癌细胞SGC-7901SGC-7901增值作用的影响及对其产生的炎症因子的改善作用。方法培养人胃癌细胞SGC-7901,用LPS诱导SGC-7901细胞,用MTS法检测不同浓度的LPS对SGC-7901细胞活力的影响,用浓度为250、50、10 mg/m L益气复生方作用于细胞,采用ELISA法检测其细胞上清液中炎症因子的释放量。结果益气复生方能够显著降低SGC-7901SGC-7901细胞的存活率,对胃癌细胞起到很好的抑制作用,能够显著改善LPS诱导的胃癌细胞肿瘤坏死因子(TNF-α)、白介素6(IL-6)、白介素8(IL-8)炎症因子的释放,能够促进干扰素(IFN-γ)的释放量。结论益气复生方可以抑制LPS刺激的SGC-7901SGC-7901细胞炎症因子的释放,对胃癌细胞具有一定的改善作用。
Objective To investigate the effect of Yiqi Fusheng Decoction on the proliferation of LPS induced gastric cancer cell line SGC-7901SGC-7901 and the improvement of its inflammatory factors. Methods Human gastric cancer cell SGC-7901 was cultured, and SGC-7901 cells were induced by LPS. The effect of different concentrations of LPS on the viability of SGC-7901 cells was detected by MTS. The cells were treated with 250, 50, and 10 mg/mL of Yiqi Fusheng Decoction, and the release of inflammatory cytokines in the supernatant was detected by ELISA. Results Yiqi Fusheng Decoction could significantly reduce the survival rate of SGC-7901SGC-7901 cells and played a good role in inhibiting gastric cancer cells. It could significantly improve the release of inflammatory factors of tumor necrosis factor （TNF-a）, interleukin 6 （IL-6） and IL-8 induced by LPS in gastric cancer cells, and could promote the release of interferon （IFN-y）. Conclusion Yiqi Fusheng Decoction can inhibit the release of inflammatory factors of SGC-7901 cells stimulated by LPS, and can improve the gastric cancer cells
作者
林燕
王艳春
段春燕
袁凤娟
LIN Yan;WANG Yan-Chun;DUAN Chun-Yan;YUAN Feng-Juan(The First Tumor Department, Affiliated Traditional Chinese Hospital, Xinjiang Medical University, Urumqi 830000, China;Xinjiang Uygur Autonomous Region for Food and Drug Inspection Institute Urumqi 830002, China)
出处
《食品安全质量检测学报》
CAS
2018年第9期2016-2019,共4页
Journal of Food Safety and Quality
基金
国家自然科学基金项目(81760860)
新疆维吾尔自治区自然科学基金项目(2016DOIC136)~~
关键词
益气复生方
胃癌细胞
炎症因子
Yiqi Fusheng Decoction
gastric cancer cell
inflammatory factor
