摘要
目的:探讨DNA甲基化在五味子乙素(Schisandrin B,Sch B)抗Aβ_(1-42)所致阿尔茨海默病中的作用及机制。方法:MTT法检测细胞存活率;Real-time PCR检测细胞中DNMT1和DNMT3A的mRNA表达;Western blot检测细胞中DNMT1和DNMT3A的蛋白含量。结果:Sch B对Aβ_(1-42)损伤的SH-SY5Y细胞具有明显改善作用,无论在细胞形态还是活力上均发生显著变化;Real-time PCR及蛋白印迹的结果显示,AD细胞模型组DNMT3A及DNMT1的mRNA和蛋白表达量均明显降低,经Sch B治疗后,各剂量给药组DNMT3A和DNMT1的mRNA和蛋白表达量显著增加。结论:Sch B能够有效抑制Aβ_(1-42)对SH-SY5Y细胞的损伤,且这可能与DNA甲基化有关。
Objective： To investigate the role and mechanism of DNA methylation in Sch B against Alzheimer＇s disease induced by Aβ（1-42）. Methods： The cell viability was detected by MTT assay. The mRNA expressions of DNMT1 and DNMT3 A were detected by realtime PCR. The protein levels of DNMT1 and DNMT3 A were detected by western blot. Results： Sch B could significantly improve the Aβ（1-42）-injured SH-SY5 Y cells, both in cell morphology and in vitality. The results of real-time PCR and Western blotting showed that the expression levels of DNMT3 A and DNMT1 mRNA and protein were significantly increased after Sch B treatment. Conclusion： Sch B can effectively inhibit the injury of SH-SY5 Y cells induced by Aβ（1-42）, and this may be related to DNA methylation.
出处
《中医临床研究》
2018年第10期1-4,共4页
Clinical Journal Of Chinese Medicine
基金
国家自然科学基金项目(NO.81560195)
内蒙古自治区卫生计生委医疗卫生计生科研计划项目(NO.201701079)
