摘要
用表面张力法测定不同水/土比下吐温80和十二烷基苯磺酸钠不同比例混合的临界胶束浓度;用响应面曲面法设计实验,确定吐温80浓度、十二烷基苯磺酸钠浓度和水/土比3个因素作用于固定化微生物降解菲的最佳组合。结果表明,相同表面活性剂在不同水/土比下的临界胶束浓度不同,水/土比越小,临界胶束浓度越大;相同水/土比时不同混合比的混合表面活性剂的临界胶束浓度不相同;响应面模型得到固定化微生物降解不同水/土比中多环芳烃菲的最优实验条件是十二烷基苯磺酸钠摩尔浓度0.014mol/L,吐温80质量浓度0.050g/L,水/土比10/1(mL/g),此时,固定化微生物降解菲的效率达到55%,十二烷基苯磺酸和吐温80的质量比为9.76/1,可用于指导混合表面活性剂强化固定化微生物修复多环芳烃污染的土壤。
The critical micelle concentration of the mixed surfactants at different ratios was confirmed by means of measuring the surface tension.Through designed experiment with response surface method,the effects of three factors,including Tween 80,sodium dodecyl benzene sulfonate and water/soil ratio,on the biodegradation of phenanthrene by immobilized microbes were executed.Results indicate that the critical micelle concentrations of surfactant at various water/soil ratios are different.The critical micelle concentration is inversely proportional to the water/soil ratio.At the same water/solid ratio,different proportions of surfactant lead to different critical micelle concentrations.The optimal experimental condition of the response surface model for the best immobilized microbes biodegradation of phenanthrene at different water/soil ratios was obtained,when the sodium dodecyl benzene sulfonate molar concentration of 1.40 mol/L,Tween80 mass concentration of 0.050 g/L,water/soil ratio at 10/1（mL/g）.Under the optimized conditions,the immobilized microorganism biodegradation rate of phenanthrene reaches 55%,and the mass ratio of sodium dodecyl benzene sulfonate to Tween 80 is 9.76/1.This result is beneficial to strengthen the remediation of polycyclic aromatic hydrocarbons contaminated soil through adding mixed surfactants at an appropriate proportion by immobilized microorganism biodegradation.
作者
尚琼琼
张秀霞
李振伟
张博凡
熊鑫
SHANG Qiongqiong,ZHANG Xiuxia,LI Zhenwei,ZHANG Bofan,IONG Xin(Department of Environmental and Safety Engineering,China University of Petroleum,Qingdao 266580,Chin)
出处
《石油学报（石油加工）》
EI
CAS
CSCD
北大核心
2018年第3期574-580,共7页
Acta Petrolei Sinica(Petroleum Processing Section)
基金
山东省自然科学基金项目(ZR2014BM023)
青岛市科技发展指导计划项目(KJZD-12-65-jch)
中国石油大学(华东)创新工程(YCX2017048)
中央高校基本科研业务费专项资金(14CX06101A)资助
关键词
固定化微生物
混合表面活性剂
多环芳烃
菲
immobilized microbes
mixed surfactants
polycyclic aromatic hydrocarbons
phenanthrene
