摘要
为了解山羊干扰素刺激基因（stimulator of interferon genes，STING）及其编码蛋白的生物学信息，本试验采用RT-PCR法克隆了海南黑山羊STING基因并进行序列及其编码蛋白的分析，构建了pEGFP—N1-STING袁达载体并转染至OFTu细胞进行了表达。结果表明STING基因的物种内同源性高、种间同源性低，编码蛋白由378个氨基酸组成，存在4个潜在跨膜区，含有1个信号肽；转染和Western blot试验证实，构建的表达载体可在OFTu细胞中成功表达。本试验为构建STING专性表达细胞系及深入研究其在山羊抗感染免疫的机制奠定了基础。
To investigate the stimulator of interferon genes（STING） of Hainan black goat and the biological information of its encoding protein,the STING gene was cloned by RT-PCR and the sequence and encoded protein were analyzed. The expression vector of pEGFP-N1-STING was constructed and transfected into OFTu cells for expression analysis. Results of nucleotide homology analysis showed that the STING gene were conservative within species while highly variant between species. The STING protein is composed of 378 amino acids with 4 potential transmembrane domains which containe one signal peptide. Transfection and Western blot experiments confirmed that the expression vector could be successfully expressed in OFTu cells. These results provided the basic data for construction of STING-specific expression cells lines and further investigation of its anti-infection mechanism of STING in goat.
作者
刘健新
彭斐
黎振标
任旭皎
许古明
葛士坤
宁章勇
LIU Jian-xin, PENG Fei, LI Zhen-biao, REN Xu-jiao, XU Gu-ming, GE Shi-kun, NING Zhang-yong(College of Veterinary Medicine, South China Agricultural University ,Guangzhou 510642, Chin)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第5期1013-1018,共6页
Chinese Journal of Veterinary Science
基金
广东省科技计划资助项目(2016A020210079)
