摘要
探讨如何提高生物样品电镜图像的反差。通过改良传统染色方法、控制样本体积、把握染液pH值及温度、改进水洗工具、喷涂碳离子、碳膜捞片、减少切片厚度、选择小孔径光阑、降低加速电压、选择正确的欠焦等多方面因素,分析增加图像反差的方法。相同倍率下,采用提高电镜图像反差的办法能够得到更为清晰的超微结构。只有注意到每一个实验操作环节,才能得到较好的图像反差效果。
To explore the effective way to improve the contrast of electron microscopic images of biological samples, a lot of methods, such as adjusting the traditional dyeing method, controlling the sample volume, regulating the pH value and temperature of the dye solution, improving the water washing tool, spraying carbon ions, using the carbon film fillet, reducing the slice thickness, selecting the smaller aperture, lowering the acceleration voltage, selecting the correct under focus and were tested to increase contrast ratio of the image. It was found that the improving methods could get a clearer uhrastructure with the same magnification, and only each experimental operation is noted, then we can get a better effect of the contrast image.
作者
刘湘花
张俊霞
王会丽
李晓冰
LIU Xianghua;ZHANG Junxia;WANG Huili;LI Xiaobing(Pathological Experiment Center;Basic Medical College, Henan University of Chinese Medicine, Zhengzhou 450046, China)
出处
《实验室研究与探索》
CAS
北大核心
2018年第3期8-9,13,共3页
Research and Exploration In Laboratory
基金
国家自然科学基金(81603527)
河南省高等学校重点科研项目(16A360010)
河南省科技攻关计划项目(162102310466)
郑州市科技攻关计划(20150310)
河南中医学院基本科研业务费(2014KYYWF-QN10)
河南中医学院科技创新人才(2015XCXRC05)
2016年度河南中医药大学大学生创新学习项目(CXXM【2016】0148)
