摘要
目的构建人胃癌原位移植转移动物模型,并利用活体成像系统对肿瘤转移进行可视化的实时监测评价。方法构建带有绿色荧光蛋白序列和萤火虫荧光素酶序列的慢病毒重组表达质粒;慢病毒包装感染人BGC-823胃癌细胞株,筛选得到BGC-823-e GFP-luc2稳转株;细胞接种高度免疫缺陷小鼠腋下,建立人胃癌皮下种植动物模型,继而建立人胃癌肿瘤皮下传代动物模型;将皮下传代模型肿瘤组织原位移植高度免疫缺陷小鼠胃部,构建人胃癌原位移植转移动物模型;利用活体成像系统对以上模型进行监测评价。结果获得稳定表达绿色荧光蛋白和萤火虫荧光素酶的BGC-823-e GFP-luc2细胞株;成功构建皮下种植和传代动物模型;成功构建人胃癌原位移植转移动物模型。结论慢病毒感染方法适用于人胃癌BGC-823-e GFP-luc2稳转株的建立;利用BGC-823-e GFP-luc2细胞株可以快速构建人胃癌皮下种植动物模型和原位移植转移动物模型,利用活体成像系统可以直观、非侵入的有效监测胃癌远端转移灶的发生、发展情况。
Objective To establish animal models of orthotopic transplantation of human gastric carcinoma and provide the visible research method to monitor tumor micrometastases with in-vivo imaging system. Methods The lentiviral expression vectors carrying luc2, e GFP and puromycin genes was constructed. BGC-823-e GFP-luc2 stable strain was screened after BGC-823 cell line was infected by the lentivirus. Then animal models with subcutanious implatation and orthotopic transplantation were established. Results BGC-823-e GFP-luc2 cell stably expressing green fluorescent protein and firefly luciferase was obtained. The animal models of subcutaneous implantation and orthotopic transplantation were successfully established. Conclusions The method of lentiviral infection is suitable for the establishment of human gastric cancer BGC-823-e GFP-luc2 cell strain, which can be used to establish human gastric cancer subcutaneously and orthotopic transplantation models complicated by metastasis rapidly. The in vivo imaging system can be used to monitor the occurrence and development of distal metastasis of gastric cancer.
作者
赵广银
苏乔
李雯雯
李武国
Zhao Guangyin;Su Qiao;Li Wenwen;Li Wuguo(Animal Laboratory Center, the First Affiliated Hospital, Sun Yat-sen Uaiversity, Guangzhou 510080, China)
出处
《中华普通外科学文献（电子版）》
2018年第2期90-95,共6页
Chinese Archives of General Surgery(Electronic Edition)
基金
广东省科技计划项目(2014A030304016)
关键词
胃肿瘤
动物模型
原位移植
活体成像系统
Stomach neoplasms
Animal models
Orthotopic transplantation
In-vivo imaging system
