摘要
建立高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)同时测定豆芽中4种药物残留的方法。豆芽经酸化乙腈匀浆提取,无水硫酸镁和十八烷基硅烷(C18)粉末分散固相萃取净化,以乙酸铵溶液和乙腈为流动相,洗脱分离后,采用多反应监测(multiple reactions monitoring,MRM)检测,基质匹配标准溶液外标法定量。结果表明,赤霉素、6-苄基腺嘌呤、4-氯苯氧乙酸、2,4-滴在1.0μg/L～200.0μg/L的范围内,其浓度与各待测物的峰面积呈良好的线性关系,相关系数均大于0.999 7,样品添加回收试验的平均回收率为63.8%～119.0%,相对标准偏差为1.94%～12.34%之间(n=6)。该方法简单准确,适用于豆芽中4种药物残留的同时检测。
A method for the simultaneous determination of four drug residues（6-benzylaminour,gibberellic acid,4-chlorophenoxyacetic acid,2,4-dichlorophenoxyacetic acid）in bean sprouts by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）was developed. The samples were extracted with acetonitrile containing 5 % acetic acid,followed by clean-up using the powder of magnesium sulfate and C18. The resulting samples were separated on a C18 column,and detected by multiple reactions monitoring（MRM） under the condition of Negative mode. The results showed that the linear calibration curves were obtained in the entire range（1.0 μg/L-200.0 μg/L）for all the above rug residues,with the correlation coefficients higher than 0.999 7. For all the samples,the average spiked recoveries ranged 63.8 % from 119.0 %,and the relative standard deviations（RSDs,n =6） were in the range of 1.94 %-12.34 %. The method is easy and accurate,and can meet the requirement for the determination of four drug residues in bean sprouts.
作者
马桂娟
汤丽华
朱捷
马雪梅
MA Gui-juan;TANG Li-hua;ZHU Jie;MA Xue-mei(Ningxia Hui Autonomous Region Food Testing Center, Yinchuan 750001, Ningxia, China)
出处
《食品研究与开发》
CAS
北大核心
2018年第10期138-142,共5页
Food Research and Development
关键词
高效液相色谱-串联质谱法
豆芽
药物残留
分散固相萃取
high performance liquid chromatography-tandem mass spectrometry
bean sprouts
drug residues
dispersive solid phase extraction
