摘要
目的研究和探讨小反刍兽疫病毒(PPRV)对小鼠骨髓来源的树突状细胞(BM-DCs)成熟和分化功能的影响。方法从小鼠骨髓中分离得到DCs,经过IL-4和GM-CSF诱导成熟;流式细胞术检测DCs表面共刺激分子表达及吞噬FITC-Dextran的能力;ELISA检测细胞因子IL-6、IL-12p40、IL-1β和IL-10的表达水平。结果利用LPS和PPRV处理DCs 24 h,发现与阳性对照LPS组相比,PPRV作用后显著抑制了CD40和MHC-Ⅱ的表达(P<0.05);滴度适度的PPRV可以显著增加CD40、CD80和CD86的表达,显著升高IL-6和IL-10的分泌(P<0.05),吞噬FITC-Dextran的能力并无显著差异,各组之间也无影响,且不同比例的PPRV对DC存活率无显著影响(P>0.05)。结论 PPRV在一定滴度范围内具有潜在的促进小鼠DC成熟的功能,为免疫学研究奠定基础。
This study aims to investigate the effect of pests des petits ruminants virus（PPRV） on thematuration and differentiation of dendritic cells（DCs） derived from murine bone marrow（BM-DC）. DCs wereinduced from Balb/c mice bone marrow in the presence of rm IL-4 and rm GM-CSF, and then subjected to flowcytometry for the expression of costimulatory molecules and the ability of FITC-Dextran phagocytosis. ELISA wasapplied to detect the production of cytokines IL-6, IL-12 p40, IL-1β and IL-10. Data showed that PPRV inhibitedthe expression of CD40 and MHC-II（P0.05）, but increased the levels of IL-6 and IL-10 of DM-DCs, ascompared LPS-treated BM-DCs. Interestedly, diluted PPRV（10-1, 10-2, 10-3） promoted the expression of CD40,CD80 and CD86, as well as the secretion of IL-6 and IL-10（P0.05）, but exerted no effect on FITC-Dextranphagocytosis of BM-DCs, as compared LPS-treated BM-DCs. And the diluted PPRV at different proportion showedon significant difference in the survival rate of DC（P0.05）. From the experiment we draw the conclusion that PPRVin a certain range titer maybe has potential function to promote the maturation of BM-DC, which would lay afoundation for further immunological research.
作者
李玲霞
吴锦艳
杜国玉
冯倩
王光祥
陈研
曹小安
刘永生
刘湘涛
尚佑军
LI Lingxia;WU Jinyan;DU Guoyu;FENG Qian;WANG Guangxiang;CHEN Yan;CAO Xiao＇ an;LIU Yongsheng;SLIU Xiangtao;HANG Youjun(State Key Laboratory of Pathogen Biology of Veterinary Diseases ＆ Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences ＆ Key Laboratory of Animal Virology, Ministry of Agriculture, Lanzhou 730046, Chin)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2018年第5期369-377,共9页
Immunological Journal
基金
国家重点研发计划课题(2017YFD0500903)
国家现代肉羊产业技术体系项目(CARS-39-04B)
关键词
小反刍兽疫病毒
BM-DC
细胞因子
流式细胞术
Pests des petits ruminants virus
Bone marrow derived dendritic cells
Cytokine
Flow cytometry
