摘要
目的研究碱性成纤维细胞生长因子(bFGF)对脊髓损伤后大鼠血脊髓屏障的修复作用。方法将大鼠随机分为假手术组、模型组、bFGF干预组(80μg/kg)。BBB法评价大鼠后肢运动功能后,于术后14 d处死;HE染色、NeuN染色观察脊髓损伤神经元丢失情况;伊文思蓝、FITC-dextran法检测血脊髓屏障的通透性;Western blot检测黏附连接蛋白(p120-catenin和β-catenin)和紧密连接蛋白(occludin和claudin-5)表达。结果术后3 d开始,bFGF干预组BBB评分明显高于模型组(P<0.05);与模型组相比,术后3 d,bFGF干预组神经元丢失明显减少(P<0.05);术后1 d,bFGF干预组血脊髓屏障的通透性减少,p120-catenin、β-catenin、occludin和claudin-5蛋白表达明显上升(P<0.05)。结论 bFGF能够减少大鼠脊髓损伤后神经元的丢失,促进黏附连接蛋白和紧密连接蛋白的表达,促进血脊髓屏障的修复。
Objective To investigate the mechanisms of basic fibroblast growth factor(bFGF)promoting blood spinal cord barrier(BSCB)recovery in rats after spinal cord injury(SCI).Methods Rats were randomly divided into sham group,SCI model group,bFGF intervention group(80μg/kg).The nerve function of hind limb motor was evaluated by Basso,Beattie,and Bresnahan(BBB)scores during postoperative 14 days.Neuron loss of injured spinal cords was observed by haematoxylin and eosin(HE)staining and NeuN staining.The integrity of BSCB was investigated with Evan s Blue staining and fluorescein isothiocyanate(FITC)-dextran extravasation.The expression protein of adhesive connection protein(p120-catenin,β-catenin)and tight junction protein expression(occludin,claudin-5)were analyzed by Western blot.Results Compared with SCI model group,bFGF intervention group neuron loss decreased significantly at 3 d after injury(P<0.05);the permeability of blood spinal cord barrier obviously reduced at 1d after injury;the expressions of p120-catenin,beta-catenin,occludin,claudin-5 protein of bFGF intervention group were increased dramatically at 1 d after injury(P<0.05).Conclusions bFGF improves the recovery of BSCB in an SCI model by reducing the loss of neurons and increasing the expressions of adhesion junction proteins and tight juction proteins.
作者
张谢
周华
张宏宇
肖健
ZHANG Xie;ZHOU Hua;ZHANG Hongyu;XIAO Jian(Dept.of Pharmacy,Ningbo Medical Treatment Center Li Huili Hospital,Ningbo 315040;Dept.of Pharmacy,Wenzhou Medical College,Wenzhou 325035,China)
出处
《基础医学与临床》
CSCD
2018年第4期502-506,共5页
Basic and Clinical Medicine
基金
宁波市自然科学基金(2015A610208)
浙江省药学会医院药学专项科研项目(2014ZYY36)
