摘要
目的对荧光定量PCR法在人巨细胞病毒感染患儿不同时段尿标本检测巨细胞病毒中的应用效果进行探讨。方法选取2015年9月~2017年2月我院儿科收治的30例人巨细胞病毒感染患儿,所有患儿分别于清晨采集晨尿作为晨尿组,于其他时段采集尿液标本作为随机时段组,应用实时荧光定量PCR技术对不同时段尿标本人巨细胞病毒DNA水平进行检查,对比两组阳性检出率。结果晨尿组,27例检出人巨细胞病毒阳性,检出率为90.00%,随机时段组,20例检出人巨细胞病毒阳性,检出率66.67%,晨尿组明显高于随机时段组,差异有统计学意义(P<0.05)。结论对人巨细胞病毒感染患儿应用实时荧光定量PCR技术检测巨细胞病毒,采集患儿晨尿作为检测标本,阳性检出率较高,可有效降低漏诊情况,在临床中值得推广应用。
Objective To investigate the effect of fluorescent quantitative PCR in detection of cytomegalovirus（HCMV）in urine samples of children with HCMV infection at different time intervals.Methods A total of 30 cases of infection in our hospital from September 2015 to February 2017 in Department of Pediatrics of infected children,for all children were collected in the morning urine as morning urine group,collecting urine specimens as random specimens from other time periods,the application of real-time fluorescence quantitative PCR technology in different periods of urine HCMV DNA level standard for examination,the positive detection rate was compared between the two groups.Results The results showed that morning urine group,27 cases were detected HCMV positive,the detection rate of 90.00%,random time urine group,20 cases were detected HCMV positive,the detection rate of 66.67%,compared the two groups,morning urine group was significantly higher than that of random time group,the difference was statistically significant（P〈0.05）.Conclusion The detection of HCMV real-time fluorescent quantitative PCR in children with infection of HCMV in children,collection of morning urine samples for testing,the positive rate is high,effectively reduce the misdiagnosis,it is worth in clinical application.
作者
黄斌
刘素云
万丽佳
刘明
吴小秧
罗勤
HUANG Bin;LIU Su-yun;WAN Li-jia;LIU Ming;WU Xiao-yang;LUO Qin(Department of Pediatrics,People＇s Hospital of Pingxiang City,Jiangxi Province,Pingxiang 337000,Chin)
出处
《中国当代医药》
2018年第12期119-121,共3页
China Modern Medicine
