摘要
目的探讨血清人巨细胞病毒(HCMV)-IgM和尿液HCMV-DNA检测对婴、幼儿HCMV感染临床诊断的意义。方法选择67例确诊为HCMV感染的婴、幼儿,分别采用酶联免疫吸附测定(ELISA)、荧光定量聚合酶链反应(PCR)检测血清HCMV-IgM及尿液HCMV-DNA。结果 HCMV感染的临床表现以肺炎和肝功能异常多见。67例确诊患儿中,血清HCMVIgM检测的阳性检出率为71.6%,尿液HCMV-DNA检测的阳性检出率为88.1%。二者联合检测,其阳性检出率为95.5%。结论血清HCMV-IgM和尿液HCMV-DNA联合检查可提高临床诊断的准确性,有助于早期诊断。
Objective To explore the significance of serum human cytomegalovirus （HCMV）-IgM and urine HCMV-DNA testing for clinical diagnosis of HCMV infection in infants and young children. Methods Sixty seven infants and young children with definite diagnose of HCMV infection were selected. Enzyme-linked immunosorbent assay（ELISA） and fluorescent quantitative poly- merase chain reaction（PCR） were employed to detect serum HCMV-IgM and urine HCMV-DNA,respectively. Results The major clinical manifestations of HCMV infection were pneumonia and liver function abnormalities. In 67 children with confirmed diagnosis,the positive detection rate of serum HCMV-IgM detection was 71.6% and that of urine HCMV-DNA was 88. 1%. When the two methods were combined,its positive detection rate was 95.5 %. Conclusion The joint detection of serum HCMV-IgM and urine HCMV-DNA can improve the accuracy of clinical diagnosis and will be conducive to early diagnosis.
出处
《国际检验医学杂志》
CAS
2013年第21期2848-2849,共2页
International Journal of Laboratory Medicine
