摘要
目的:建立射干扶正口服液中射干苷的含量测定方法。方法:采用高效液相色谱法(HPLC),色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm),以乙腈-水(28:72)为流动相,流速为1.0 m L/min,检测波长265 nm,柱温30℃进行检测。结果:射干苷的线性范围为:18.40~184.00μg/m L,线性方程为:Y=44.21X-0.6,r=0.999 8,r=0.999 8;平均加样回收率为99.75%(RSD%=1.11%)。结论:该法稳定、快速、简便,结果准确、可靠,可用于射干扶正口服液中射干苷的含量测定。
Objective： To establish the content determination method of belamcandin in She Gan Fu Zheng oral liquid. Methods： HPLC was adopted when chromatographic column was Agilent Eclipse Plus C18（250 mm ×4.6 mm, 5 μm）, methyl cyanide-water（28 ∶72） was taken as mobile phase, flow rate： 1.0 m L/min, detection wavelength： 265 nm, column temperature： 30℃. Results： Linearity range of belamcandin was 18.40～184.00 μg/m L,linear equation was： Y=44.21 X-0.6, r=0.999 8, r=0.999 8, average recovery rate was 99.75%（RSD% =1.11%）.Conclusion： The method, stable, rapid, simple, accurate and reliable, could be used for content determination of belamcandin in She Gan Fu Zheng oral liquid.
作者
王红丽
展锐
郭敏
WANG Hongli;ZHAN Rui;GUO Min(Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Chin)
出处
《西部中医药》
2018年第4期35-36,共2页
Western Journal of Traditional Chinese Medicine
基金
甘肃省中医药管理局课题(编号GZK-2014-65)
