摘要
为探究汉黄芩苷是否具有抗衰老作用,本研究以果蝇为模型,考察汉黄芩苷对果蝇自然寿命的影响。采用RT-PCR和UPLC-MS/MS代谢组学技术,探索汉黄芩苷发挥抗衰老作用的潜在机制。结果显示,0.02和0.5 mg/mL汉黄芩苷均可整体延长果蝇寿命,并能够分别延长果蝇平均寿命5.64%和5.39%,延长最高寿命2.74%和5.12%;与30 d组相比,汉黄芩苷能够显著上调果蝇体内抗氧化酶基因SOD1、SOD2和CAT的表达水平,下调MTH的表达水平。果蝇代谢组学分析共找到17个潜在生物标志物,主要参与氨基酸代谢(D-谷氨酰胺和D-谷氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,精氨酸和脯氨酸代谢,缬氨酸、亮氨酸和异亮氨酸代谢)和能量代谢(氮代谢)。该结果表明,汉黄芩苷延缓衰老与上调抗氧化基因表达和调控不同代谢途径有关。
To investigate whether wogonoside has an anti-aging role,we studied its effect on the natural lifespan of Drosophila melanogaster. Metabonomics techniques,such as RT-PCR and UPLC-MS/MS,were used to explore the potential anti-aging effect of wogonoside. The results showed that 0. 02 mg/mL and 0. 5 mg/mL wogonoside could significantly prolong the overall longevity of fruit flies and improve the mean lifespan by 5. 64% and 5. 39%,and the maximum lifespan by 2. 74% and 5. 12%,respectively.Dietary supplement of wogonoside significantly up-regulated the expression of antioxidant genes SOD1,SOD2 and CAT,and down-regulated the expression of MTH in fruit flies,compared with the 30 d group.Seventeen potential biomarkers regulated by wogonoside treatment were found. The metabolic pathwayanalysis suggested that wogonoside antagonizes senescence mainly through modulating amino acid metabolism（ D-glutamine and D-glutamate metabolism; alanine,aspartate and glutamate metabolism;arginine and proline metabolism; valine,leucine and isoleucine biosynthesis） and energy metabolism（ nitrogen metabolism）. The results suggest that the anti-aging role of wogonoside is associated with increasing the expression of antioxidant genes and regulating different metabolic pathways.
作者
薛立英
高丽
秦雪梅
杜冠华
周玉枝
XUE Li-Ying;GAO Li;QIN Xue-Mei;DU Guan-Hua;ZHOU Yu-Zhi(Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;National Drug Screening Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2018年第4期404-416,共13页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金项目(No.81673572)
山西省重点研发计划项目(No.201703D321023-3)
山西省应用基础研究项目(No.201601D021164)
山西省高校科技创新项目(No.2016120)
山西省科技基础条件平台建设项目(No.2014091022)资助~~
