摘要
目的:评价高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)黄七胶囊中黄芪甲苷的含量测定。方法:以十八烷基硅烷键合硅胶为填充剂;以乙腈-水(32∶68)为流动相;蒸发光散射检测器;柱压11.2~12.3 MPa;流速:1.0 m L/min;柱温:室温。理论塔板数以黄芪甲苷峰计算应不低于4 000;峰拖尾因子为1.02。结果:HPLC-ELSD法精密度、重复性、稳定性、回收率良好。黄芪甲苷对照品进样量在1.0528~10.528μg范围内线性关系良好(r=0.999 9,n=7)。结论:高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷含量是可行的。
Objective： To evaluate contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD. Methods： The octadecyl silane bonding silica gel was the filler,with acetonitrile-water（ 32∶ 68） as mobile phase,and the detector was ELSD with the column pressure of 11. 2 ~ 12. 3 MPa and the flow rate of 1. 0 m L/min. Column temperature： indoor temperature. Theoretical plate number was calculated by puerarin peak which should not be less than 4 000; Trailing factor was 1. 02. Results： The method of HPLC-ELSD had good accuracy,repeatability,stability and recovery rate. A good linearity relationship of the sample size of asreagaloside comparison products in the range of 1. 0528-10. 528 μg（ r = 0. 999 9,n = 7）. Conclusion： Contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD is feasible.
作者
杜鹏
顿宝生
杨新杰
彭修娟
Du Peng;Dun Baosheng;Yang Xinjie;Peng Xiujuan(Shaanxi Medical Institutions and Drug Procurement Center, Xi＇an 710065, China;Chinese Medicinal Research Institute, Shaaaxi Institute of International Trade ＆ Commerce, Xianyang 712046, China;Shaanxi Institute of Traditional Chinese Medicine, Xianyang 712046, China)
出处
《世界中医药》
CAS
2018年第2期460-463,共4页
World Chinese Medicine
基金
咸阳市2014年重大科技专项(2014k01-21)
