摘要
目的研究PTEN干扰对甲状腺滤泡上皮细胞Nthy—ori3—1侵袭能力的影响，并初步探讨其可能的分子机制。方法以人正常甲状腺滤泡上皮细胞Nthy—ori3—1为研究对象，通过慢病毒感染技术将PTEN特异性短发夹RNA（shRNA）及无关序列shRNA导入细胞。实验分为2组：无关序列组（shContr01）和干扰PTEN组（shPTEN）。通过Transwell小室侵袭实验检测细胞的侵袭能力，免疫荧光检测侵袭相关蛋白在细胞表面的表达水平，Westernblot检测相关蛋白的表达水平。结果与无关序列组相比，干扰PTEN组细胞的形态发生明显改变，侵袭相关蛋白E—cadherin的表达水平明显降低，细胞的侵袭能力增强，侵袭相关蛋白E—cadherin在细胞表面的表达水平降低。结论PTEN干扰能够促进甲状腺滤泡上皮细胞Nthy—ori3—1的侵袭，且这一作用可能是通过下调侵袭相关蛋白E—cadherin的表达实现的。
Objective To investigate the effects of PTEN interference on the invasive ability of thyroid follicular epi- thelial cells and to explore its possible molecular mechanism. Methods A human normal thyroid follicular epithelial cell line （ Nthy - ori3 - 1 ） was used, to which PTEN - targeted short hairpin RNA （shRNA） and unrelated sequence - targe- ted shRNA were transfected by lentivirus. There were two groups in the experiments： an unrelated sequence （shControl） group and a PTEN interference （shPTEN） group. The invasion ability of ceils was detected by Transwell cell invasion as- say. The level of invasion related proteins were detected by immunofluorescence. The level of related proteins were detec- ted by Western blotting. Results Compared with the shControl group, the shPTEN group showed remarkable morpholog- ical changes and marked enhanced invasive abilities, with significantly reduced levels of E - cadherin, especially on the cell surface. Conclusions PTEN interference can promote the invasion of Nthy - ori3 - 1 cells, which may be mediated by down - regulating expression of E - cadherin.
作者
卢今奇
吴牧雨
李明岩
付家驹
孙卓
吴永平
LU Jinqi;WU Muyu;LI Mingyan;FU Jiaju;SUN Zhuo;WU Yongping(Department of Pathology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China)
出处
《徐州医科大学学报》
CAS
2018年第3期141-145,共5页
Journal of Xuzhou Medical University
基金
国家自然科学基金（81372479,81502387）
徐州市科技创新计划（KC15SH069）
江苏省高校研究生创新计划项目（KYLX16-1134）
