摘要
【目的】探讨不同病变评分痒螨病患兔皮损中细胞因子IFN-γ、IL-4和转录因子T-bet、GATA-3的表达。【方法】采用免疫组化、酶联免疫吸附试验和荧光定量PCR法检测痒螨病患兔(病变评分为1分、3分和5分)耳部皮损和健康兔(0分)耳部皮肤中IFN-γ/IL-4和T-bet/GATA-3的表达。【结果】与0分组相比,患病组(1分、3分和5分组)皮损处IFN-γ蛋白和IFN-γmRNA均增高,但仅3分组呈显著增高(IFN-γ蛋白:0分组=(0.344 2±0.014 33)pg/mg,3分组=(0.463 6±0.014 95)pg/mg,P<0.05;IFN-γmRNA:3分组/0分组=2.365,P<0.05)。患病组皮损处T-bet和GATA-3蛋白的阳性产物均定位于细胞核,但两者较健康组均无明显差异(P>0.05);1分组和3分组皮损中的T-bet mRNA较健康组呈显著增高(P<0.05),而3分组和5分组皮损中GATA-3 mRNA呈显著降低(P<0.05)。【结论】随着痒螨病病变评分的增加,宿主的Th2免疫反应受到抑制(GATA-3的表达降低),而Th1免疫反应则有所增强(IFN-γ和T-bet的表达增高)。
【Objective】The purpose of this study is to investigate the expression levels of cytokine factorsIFN-γ/IL-4 and transcription factors T-bet/GATA -3 in psoroptic mange with different lesion scores inrabbits.【Method】The expression levels of IFN-γ/IL-4 and T-bet/GATA-3 in ear skin samples of rab-bits among psoroptic mange groups （lesion score 1 group, score 3 group and score 5 group） and healthycontrol group （score 0 group） were investigated by immunohistochemistry, ELISA and RT-PCR.【Result】Compared to score 0 group, the protein and mRNA level of IFN-γ both increased in psoroptic mangegroups （score 1 group, score 3 group and score 5 group）, but there was significant difference betweenscore 0 group [IFN-γ protein： （0.3442±0.014 33） pg/mg] and score 3 group [IFN-γ protein： （0.4636±0.014 95）pg/mg, IFN-γ mRNA： score 3 group/ score 0 group=2.365; P〈0.05]. Both T-bet and GATA-3 were found原ed in the nucleus, but there was no significant difference in the levels of T-bet protein and GATA-3protein between psoroptic mange groups and the healthy group（P〉0.05）. However, compared with score0group, the levels of T-bet mRNA were significantly increased in the score1 and score3 lesion groups（P〈0.05）, and the level of GATA -3 mRNA was significantly decreased in the score3 and score5 lesiongroups（P〈0.05）.【Conclusion】Our results indicated that psoroptic mange rabbits elicited an inhibition ofTh2 immune response and enhancement to Th1 immune response with the increases of the skin lesionscores, showing the levels of a decrease of GATA-3 and an increase of IFN-γ and T-bet.
作者
廖晓霞
赵习彬
赖为民
谢跃
杨光友
古小彬
LIAO Xiao-xia;ZHAO Xi-bin;LAI Wei-min;XIE Yue;YANG Guang-you;GU Xiao-bin(College of VeterinaryMedicine,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2018年第2期247-253,共7页
Journal of Sichuan Agricultural University
基金
四川省教育厅项目(14ZB0003)
