摘要
为研究蜡蚧菌与新型生物农药混用在生物防治中的应用,本文采用PDA培养基培养蜡蚧菌BNCC336750分生孢子。采用浸渍法对大豆蚜进行毒力测定,选取最佳防治效果的108孢子/m L的孢子悬浮液浓度与亚致死浓度的化学农药35%吡虫啉悬浮剂和生物农药0.3%印楝素乳油、0.5%苦参碱水乳剂进行混配。结果表明,大豆蚜的累计校正死亡率随菌株孢子悬浮液浓度的增大而增大,当浓度为108孢子/m L时对大豆蚜毒力最高。菌药混用对大豆蚜的防治效果比单用蜡蚧菌BNCC336750孢子悬浮液或亚致死浓度生物农药的防治效果都要好。其中菌株BNCC336750孢子悬浮液与印楝素混用效果最好,处理7 d后大豆蚜累计死亡率达到94.16%。与苦参碱和吡虫啉混用7 d后大豆蚜累计死亡率分别为86.13%和80.29%。田间试验表明,印楝素混配菌株BNCC336750孢子悬浮液效果最好,7 d后防治效果达到89.47%。
To explore the application of Lecanicillium lecanii in combination with biological pesticides against the Aphis glycines, we cultured the conidia of L. lecanii BNCC336750 with PDA media and measured their toxicity to A. glycines by using leaf dipping method in laboratory. A spore suspension of higher toxicity obtained through bioassway as mixed with sub-lethal concentrations of three pesticides. The results indicated that the mortality of A. glycines increased with the increase of conidia liquid concentration of L. lecanii, the highest toxicity was reached at 108 spores/m L. The laboratory results showed a synergistic effect on the control efficacy of the mixture over that obtained with spore solution or sub-lethal pesticides solutions alone. Among the pesticides, nimbin mixed L. lecaniihad the highest control, with cumulative corrected mortality of 94.16% at the 7 th day. The cumulative corrected mortalities of matrine and imidachloprid mixed with L. Lecanii were 86.13% and 80.29% at the 7 th day, respectively. Field experiments showed a highest control of 89.47% with the mixtures of L. lecanii and nimbin at the 7 th day. Therefore, low concentration insecticides mixed with L. lecanii had good control against A. glycines, thus reducing the amount of pesticides substantially.
作者
莽逸伦
韩岚岚
赵奎军
李东坡
MANG Yilun;HAN Lanlan;ZHAO Kuijun;LI Dongpo(College of Agriculture, Northeast Agricultural University, Harbin 150030, China)
出处
《中国生物防治学报》
CSCD
北大核心
2018年第2期266-273,共8页
Chinese Journal of Biological Control
基金
现代农业产业技术体系(CARS-04)
关键词
蜡蚧菌
生物防治
防治效果
大豆蚜
Lecanicillium lecanii
biological control
control effect
Aphis glycines
