摘要
酿酒酵母ADR1基因是编码过氧化物酶蛋白质转录的正向调节基因,对乙醇代谢有正向调节作用。本文采用PCR技术首次在贝酵母基因组DNA中对ADR1基因序列进行全长克隆,利用在线分析工具Prot Param、Prot Scale、TMHMM、Predict Protein、Swiss-Model等软件对其编码蛋白质的基本理化性质进行分析,同时预测了该基因所编码蛋白质的二级结构和三级结构。结果表明:该核苷酸序列含有一个长3960 bp的开放阅读框,可编码1319个氨基酸。编码的蛋白质为在细胞核中行使调控功能的亲水蛋白,含有17个丝氨酸(S)激酶潜在磷酸化位点、四个coil区和2个锌指结构域,与酿酒酵母ADR1基因所编码的蛋白质结构和性质极为相似,可初步认为贝酵母ADR1基因是乙醇脱氢酶的调控基因。
The ADRI gene of Saccharomyces cerevisiae is a positive regulator of transcription of genes encoding peroxisomal proteins.To regulate alcohol metabolism,it has a positive effect.The full-length sequence of ADR1 gene was cloned from the genomic DNA of Saccharomyces bayanus using PCR for the first time.The ADRI gene of S.bayanus was cloned,and the physical and chemical properties of ADR1 protein were analyzed by online analytical tools, such as ProtParam, ProtScale, TMHMM, PredictProtein,and Swiss-Model. At the same time, the protein secondary structure and tertiary structures of protein were predicted.The results indicated that the ADR1 genc contained an opening reading frame （ORF）of 3960 bp encoding 1319 predicted amino acids.The protein was a hydrophilic protein exercising their control functions in the nucleus. It contained 17 serine kinase potential phosphorylation sites,4 coil domains, and two zinc finger domains.The protein encoded by ADR1 gene of S.cerevisiae was extremely similar to the predicted protein. It inferred that ADR1 gene of S.bayanus might play a role in regulating alcohol dehydrogenase gene.
作者
张先昂
贺笑
刘小珍
叶琴霞
黄彩双
文丽辉
张汉尧
ZHANG Xian-ang1,HE Xiao2 ,LIU Xiao-zhen1,YE Qin-xia1, HUANG Cai-shuang1, WEN Li-hui1, ZHANG Han-yao1(1.Key Laboratory for Forest Genetic and Tree Improvement ＆ Propagation in Universities of Yunnan Province in Southwest Forestry University, Kunming 650224, China 2.College of Life Sciences in Anhui Normal University,Wuhu 241000,Chin)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第7期90-96,共7页
Science and Technology of Food Industry
基金
云南省高校林木遗传改良与繁育重点实验室开放基金资助(YNGBT20106)
云南省林学一流学科建设经费(51600625)
关键词
贝酵母
ADR1
乙醇代谢
生物信息学方法
Saccharomyces bayanus
ADR1
alcohol metabolism
method of bioinformatics
