摘要
目的:克隆贝酵母SSU1基因,对其进行序列分析。方法:采用PCR技术首次在贝酵母基因组DNA中对SSU1基因序列进行全长克隆,用NCBI、ExPASy等网络数据库和ANTHEPROT等分子生物学软件分析其序列,对其功能进行预测,建立基于SSU1同源基因的系统发育树,对其分子进化进行分析。结果:克隆得到贝酵母SSU1基因,全长872 bp,包含1个387 bp的开放阅读框,编码126个氨基酸,其疏水性大于亲水性。编码的蛋白质不存在信号肽及卷曲螺旋结构,而含有跨膜区域。该蛋白为单链蛋白,主要由无规则卷曲和α-螺旋构成。基因编码的蛋白含有1个亚硫酸盐敏感蛋白SSU1及其运载体蛋白酶的TDT家族和1个TehA功能域。贝酵母与巴斯德酵母遗传关系较近。结论:贝酵母SSU1基因编码的质膜蛋白为疏水性转运蛋白,具有调节亚硫酸盐代谢的功能,是酿酒酵母中1个重要的亚硫酸盐调控基因。
Objective： The aim was cloning and analysing the sequence of SSU1 gene obtained from Saccharomyces bayanus. Method： A full-length sequence of SSU1 gene was cloned from the genomic DNA of Saccharomyces bayanus using PCR. The sequence and function of it was analyzed using net work database, such as NCBI, Expasy, and molecular biology software such as ANTHEPROT. A phylogeny tree was developed based on homologous genes about the SSU1, and the phylogenetic relationship of it was analyzed. Result： The results of this study indicated that the SSU1 gene is 872 bp in full length and contains an opening reading frame （ORF） of 387bp encoding a 126 predicted amino acids, which was encoded for a kind of hydrophobic single-chain protein without signal peptide and a coiled-coil structure, containing a transmembrane region, composed of random coil and a-helix, containing a contain sulfite sensitive protein SSU1 and its carrier protein of the TDT family and one TehA domain, and the genetic distances between S. bayanus and S. pastorianus strains were a little bit small. Conclusion： The SSU1 gene of Saccharomyces plays an important role in regulating sulfite metoblism.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2014年第9期195-201,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
教育部留学回国人员科研启动基金资助项目
农业部农业微生物资源收集与保藏重点实验室开放基金
关键词
贝酵母
SSU1
序列分析
Saccharomyces bayanus
SSU1
sequencing analysis
