摘要
目的探讨广西壮族人群磷酸二酯酶4D(PDE4D)基因rs966221位点单核苷酸多态性与缺血性脑卒中(IS)发病的关系。方法采用病例-对照研究设计,运用SNaPshot技术对101例广西壮族IS患者(IS组)及同期104例健康体检者(对照组)的PDE4D基因rs966221位点单核苷酸多态性进行检测,比较2组各基因型及等位基因频率,并进行相关性分析。结果 IS组与对照组PDE4D基因rs966221位点GG、GA、AA基因型(0.99%vs 3.85%,29.70%vs 21.15%,69.31%vs 75.00%)及G、A等位基因频率(15.84%vs 14.42%,84.16%vs 85.58%)比较,差异无统计学意义(P>0.05)。单因素及多因素logistic回归分析显示,在显性(AAvs GG+GA)、隐性(GGvs AA+GA)和加性(GGvs AA)3种遗传模型下,PDE4D基因rs966221位点单核苷酸多态性与IS患病风险均无关联(P>0.05)。结论广西壮族人群PDE4D基因rs966221位点单核苷酸多态性与IS发病不相关。
Objective To study the relationship between phosphodiesterase 4 D（PDE4 D）gene rs966221 single nucleotide polymorphisms（SNPs）and ischemic stroke（IS）in Guangxi Zhuang population.Methods One hundred and one IS patients from Guangxi Zhuang Autonomous Region served as IS pgroup and 104 healthy subjects undergoing physical ecamination served as control group in this study.Their PDE4 Dgene rs966221 SNPs were detected by SNaPshot technique.The genotypes and frequencies of alleles were compared between the two groups and the relationship between PDE4 Dgene rs966221 SNPs and IS was analyzed.Results No significant difference was found in the GG,GA,AA genotypes and in the frequencies of G and A alleles between the two groups（0.99% vs 3.85%,29.70% vs 21.15%,69.31% vs 75.00%,P0.05;15.84% vs 14.42%,84.16%vs 85.58%,P0.05）.Univariate and multivariate logistic regression analysis showed that the PDE4 Dgene rs966221 SNPs were not related with the risk of IS in dominant AAvs GG＋GA,recessive GGvs AA＋GA and additive GGvs AA genetic models（P0.05）.Conclusion The PDE4 Dgene rs966221 SNPs are not related with IS in Guangxi Zhuang population.
作者
江东东
李海华
李建立
肖友生
甘露
罗曼
Jiang Dongdong, Li Haihua, Li Jianli, Xiao Yousheng, Gan Lu, Luo Man(Department of Neurology ,First Affiliated Hospital of Guangxi Medical University ,Nanning 530021, Guangxi Zhuang Autonomous Region, Chin)
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2018年第3期271-274,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
广西自然科学基金(2013GXNSFBA019131
2015GXNSF AA139171)
关键词
环核苷酸磷酸二酯酶类
4型
多态性
单核苷酸
基因频率
基因型
卒中
磁共振成像
cyclic nucleotide phosphodiesterases, type 4
polymorphism, single nucleotide
gene fre-quency
genotype
stroke
magnetic resonance imaging
