摘要
目的:探讨附子-干姜药对治疗心力衰竭的作用机制。方法:本研究首先构建心力衰竭相关的蛋白互作网络,然后选取附子-干姜药对含有的24个活性成分,预测其作用靶点,进而构建附子-干姜药对活性成分-预测靶点网络、构建与心力衰竭相关的附子-干姜药对活性成分-潜在靶点网络、进行基于京都基因与基因组百科全书(KEGG)的生物通路富集分析,研究附子-干姜药对治疗心力衰竭的作用机制。结果:与心力衰竭相关的附子-干姜药对活性成分-潜在靶点网络包含23个靶点,关键靶点涉及碳酸酐酶2(CA2),11-β-羟基类固醇脱氢酶1(HSD11B1),基质溶素-1(MMP-3),肽基脯氨酰顺反异构酶FKBP1A(FKBP1A),磷酸二酯酶4D(PDE4D),热休克蛋白90α(HSP90AA1)等。KEGG通路7条,主要涉及肾素-血管紧张素系统(Renin-angiotensin system)等。结论:本研究结果初步验证和预测了附子-干姜药对治疗心力衰竭的作用机制,并为进一步深入揭示其作用机制奠定了良好基础。
Objective： To investigate the mechanism of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma in the treatment of heart failure. Method： Protein-protein interaction network related to heart failure was constructed, and targets related to 24 active chemical components from Aconiti Lateralis Radix Praeparata- Zingiberis Rhizoma were predicted. Active compound-predicted target network, active compound-potential target network related to heart failure, and potential target-pathway network were constructed to explore the mechanism of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizonm in the treatment of heart failure. Result： The active compound-potential target network of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma related to heart failure contained 23 targets, in which key targets involved Carbonic anhydrase 2 （CA2）, Corticosteroid l l-beta- dehydrogenase isozyme 1 （HSDI1B1）, stromelysin-1 （MMP-3）, peptidyl-prolyl cis-trans isomerase FKBP1A （FKBP1A）, cAMP-specific 3, 5-cyclic phosphodiesterase 4D （ PDE4D）, and heat shock protein HSP 90-alpha （HSP90AA1）. In Kyoto encyclopedia of genes and genomes （KEGG） pathway enrichment analysis, there were 7 KEGG pathways, mainly involving Renin-angiotensin system. Conclusion： The findings preliminarily verify andpredict the basic pharmacological effects and Rhizoma in the treatment of heart failure, in Aconiti Lateralis Radix Praeparata-Zingiberis relevant mechanisms of Aconiti Lateralis Radix order to lay a solid foundation for further studies Rhizoma in the treatment of heart failure.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第21期212-219,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81473547
81673829)
关键词
附子-干姜
心力衰竭
网络药理学
蛋白互作网络
靶点
信号通路
Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma
heart failure
networkpharmacology
protein-protein interaction network
target
signaling pathway
