摘要
采用倒置相差显微镜观察法、HE染色法、扫描电镜法和透射电镜法对梁氏异蝎毒素抑制HeLa细胞增殖的形态学影响进行了较系统的研究。用不同浓度的蝎毒处理HeLa细胞(0,20,40,60μg/m L),发现随着蝎毒浓度的升高HeLa细胞的死亡率增加,经毒素作用后,试验组与对照组相比,细胞突起收缩、形态变圆、体积缩小,染色质浓缩,导致细胞大量凋亡。结果表明:梁氏异蝎毒素对HeLa细胞的形态学具有很大的影响,能显著抑制HeLa细胞增殖。该研究对利用梁氏异蝎毒素开发新型抗癌药物提供了新的信息。
In this study, effect of Heterometrus liangi toxin on the morphology of HeLa cell investiga- ted by means of the inverted microscope, scanning electron microscopy, transmission electron mi- croscopy and HE staining. The HeLa cell was treated by different concentration of Heterometrus liangi toxin solutions （0,20,40,60 μg/mL）. The results showed that the death rate of HeLa cell was increased with increasing solution concentrations. And, the treated HaLe cell showed more round, smaller, less chromatin and a large number of cell appeared apoptosis when compared with controls. In conclusion, the Heterometrus liangi toxin had a great influence on the morphology of He- La cells, and could significantly inhibit the proliferation of the cells. This result will provide new in- formation for Heterometrus liangi toxin to be developed new anticancer drugs.
作者
唐美蓉
王娟
卓俊哲
黄婷
王智
TANG Meirong;WANG Juan;ZHUO Junzhe;HUANG Ting;WANG Zhi(College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China)
出处
《经济动物学报》
CAS
2018年第1期1-5,共5页
Journal of Economic Animal
基金
湖南省科技厅重点项目(2014FJ2003)
